ICOS ligand Lysate | ICOSLG lysate

Human ICOS ligand HEK293 Overexpression Lysate

Cat. Num. AAA8119631

• Applications: Western Blot
• Synonyms: ICOS ligand; Human ICOS ligand HEK293 Overexpression Lysate; Human ICOS Ligand/B7-H2/ICOSLG HEK293 Cell Lysate (WB positive control); Human B7-h2 Overexpression Lysate; Human B7H2 Overexpression Lysate; Human B7RP-1 Overexpression Lysate; Human B7RP1 Overexpression Lysate; Human CD275 Overexpression Lysate; Human GL50 Overexpression Lysate; Human ICOS ligand Overexpression Lysate; Human ICOS-L Overexpression Lysate; Human ICOSL Overexpression Lysate; Human ICOSLG Overexpression Lysate; Human LICOS Overexpression Lysate; inducible T-cell co-stimulator ligand; ICOSLG lysate

• Host: HEK293 Cells
• Form/Format: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.; 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

• Applicable Applications for ICOSLG lysate: Western Blot (WB)
• Application Notes: Western blot (WB): Use at an assay dependent dilution.
• Species: Human
• Sequence Information: A DNA sequence encoding the human ICOSLG (NP_056074.1) extracellular domain (Met 1-Ser 258) was fused with a polyhistidine tag at the C-terminus.
• Preparation Method: Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.; 2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
• Preparation and Storage: Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Related Product Information for ICOSLG lysate

Description: This Human ICOS ligand overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ICOS ligand protein from the overexpression lysate was verified.

Background: Human Cell lysate that Human ICOSLG/B7-H2/ICOSL/CD275 transfected/overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

Product Categories/Family for ICOSLG lysate

Cancer Drug Targets

References

Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.