ARHI Cell Lysate | DIRAS3 cell lysate
Human ARHI HEK293 Overexpression Lysate
Gene Names
DIRAS3; ARHI; NOEY2
Applications
Western Blot
Synonyms
ARHI; Human ARHI HEK293 Overexpression Lysate; Human ARHI/DIRAS3 HEK293 Cell Lysate (WB positive control); DIRAS family; GTP-binding RAS-like 3; Human ARHI Overexpression Lysate; Human DIRAS3 Overexpression Lysate; Human NOEY2 Overexpression Lysate; Human RHOI Overexpression Lysate; DIRAS3 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
461aa
Sequence
Met1-Lys225
Applicable Applications for DIRAS3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human DIRAS3 (O95661) (Met1-Lys225) was expressed with the Fc region of mouse IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for DIRAS3 cell lysate
ARHI, also known as DIRAS3, belongs to the small GTPase superfamily, Di-Ras family. ARHI gene is a novel tumor suppressor gene located on chromosome 1p31. Downregulation of ARHI expression has been detected in many types of cancer. ARHI is expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers. As a suppressor, ARHI is not only an important factor in the pathogenesis of gastric cancer, but also a potential factor for tumor aggravation. ARHI expression in gastric cancer can be employed to indicate favorable prognosis for the disease.
This Human ARHI overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ARHI protein from the overexpression lysate was verified.
This Human ARHI overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ARHI protein from the overexpression lysate was verified.
References
Pei XH. et al., 2011, Cell Biol Int. 35 (10): 1019-24.Lin D. et al., 2011, J Int Med Res. 39 (5): 1870-5.Wang W. et al., 2012, Oncol Rep. 27 (6): 1953-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 25 kDa
Observed: 24 kDa
Observed: 24 kDa
NCBI Official Full Name
GTP-binding protein Di-Ras3
NCBI Official Synonym Full Names
DIRAS family, GTP-binding RAS-like 3
NCBI Official Symbol
DIRAS3
NCBI Official Synonym Symbols
ARHI; NOEY2
NCBI Protein Information
GTP-binding protein Di-Ras3; ras homolog gene family, member I; rho-related GTP-binding protein RhoI; distinct subgroup of the Ras family member 3
UniProt Protein Name
GTP-binding protein Di-Ras3
Protein Family
UniProt Gene Name
DIRAS3
UniProt Synonym Gene Names
ARHI; NOEY2; RHOI
UniProt Entry Name
DIRA3_HUMAN
NCBI Description
This gene is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]
Uniprot Description
DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]
Protein type: G protein, monomeric, di-Ras; G protein, monomeric; G protein
Chromosomal Location of Human Ortholog: 1p31
Cellular Component: plasma membrane
Molecular Function: GTPase activity; protein binding; GTP binding
Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity
Research Articles on DIRAS3
Similar Products
Product Notes
The DIRAS3 diras3 (Catalog #AAA8115355) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 461aa. AAA Biotech's ARHI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the DIRAS3 diras3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys22 5. It is sometimes possible for the material contained within the vial of "ARHI, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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