NTRK2/TRKB Active Protein | NTRK2 active protein

Human CellExp NTRK2 / TRKB, human recombinant

Cat. Num. AAA844587

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Applications: SDS-Page
• Purity: >=95% by SDS-PAGE gel
• Synonyms: NTRK2/TRKB; Human CellExp NTRK2 / TRKB; human recombinant; NTRK2; TRKB; GP145-TrkB.; NTRK2 active protein

• Host: HEK293 cells (Cys 32-His 430)
• Purity/Purification: >=95% by SDS-PAGE gel
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 477

• Applicable Applications for NTRK2 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to bind mouse BDNF in functional ELISA.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; After reconstitution, aliquot and store at -20 degree C and use within 3 months. Avoid reapeated freezeing and thawing cycles.; Shelf Life: 12 months

Testing Data

Related Product Information for NTRK2 active protein

Background: Neurotrophic tyrosine kinase receptor type 2 (NTRK2) also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is "ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate". NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

Product Categories/Family for NTRK2 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 55956790
• NCBI GeneID: 4915
• NCBI Accession #: NP_001007098.1
• NCBI GenBank Nucleotide #: NM_001007097.2
• UniProt Accession #: Q16620; Q16675; Q59GJ1; Q8WXJ5; Q8WXJ6; Q8WXJ7; B1ANZ4; B4DFV9
• Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45 kDa. The predicted N-terminus is Cys 32. DTT-reduced Protein migrates as 60-90 kDa due to glycosylation.
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor isoform b
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: postsynaptic membrane; cell surface; growth cone; rough endoplasmic reticulum; integral to plasma membrane; presynaptic active zone; dendritic spine; terminal button; excitatory synapse; perikaryon; endosome membrane; cytosol; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; retinal rod cell development; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; neuron migration; regulation of dendrite development; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; vasculogenesis; inflammatory response; neuromuscular junction development; aging; central nervous system neuron development; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system neuron development; positive regulation of phosphoinositide 3-kinase cascade; glutamate secretion; long-term memory; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Obesity, Hyperphagia, And Developmental Delay