C-Reactive Protein Cell Lysate | CRP cell lysate
Mouse C-Reactive Protein HEK293 Overexpression Lysate
Gene Names
Crp; AI255847
Applications
Western Blot
Synonyms
C-Reactive Protein; Mouse C-Reactive Protein HEK293 Overexpression Lysate; Mouse CRP/C-Reactive Protein HEK293 Cell Lysate (WB positive control); C-reactive protein; pentraxin-related; Mouse AI255847 Overexpression Lysate; CRP cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
216aa
Sequence
Met1-Ser225
Applicable Applications for CRP cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse CRP (NP_031794.3) (Met 1-Ser 225) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CRP cell lysate
C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
This Mouse C-Reactive Protein overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of C-Reactive Protein protein from the overexpression lysate was verified.
This Mouse C-Reactive Protein overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of C-Reactive Protein protein from the overexpression lysate was verified.
References
Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12. Thompson D. et al., 1999, Structure. 7(2): 169-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,360 Da
NCBI Official Full Name
C-reactive protein
NCBI Official Synonym Full Names
C-reactive protein, pentraxin-related
NCBI Official Symbol
Crp
NCBI Official Synonym Symbols
AI255847
NCBI Protein Information
C-reactive protein
UniProt Protein Name
C-reactive protein
Protein Family
UniProt Gene Name
Crp
UniProt Synonym Gene Names
Ptx1
UniProt Entry Name
CRP_MOUSE
Uniprot Description
CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; growth cone; extracellular region; filopodium
Molecular Function: protein homodimerization activity; low-density lipoprotein receptor binding; metal ion binding; complement component C1q binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding
Biological Process: protein polymerization; positive regulation of nitric oxide biosynthetic process; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; acute-phase response; negative regulation of superoxide release; complement activation, classical pathway
Research Articles on CRP
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Product Notes
The CRP crp (Catalog #AAA8116589) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 216aa. AAA Biotech's C-Reactive Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CRP crp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser22 5. It is sometimes possible for the material contained within the vial of "C-Reactive Protein, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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