CA5A active protein

CA5A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120317

• Gene Names: CA5A; CA5; CAV; CAVA
• Purity: >96% as determined by SDS-PAGE
• Synonyms: CA5A; CA5A Protein; Human; Recombinant (His Tag); Human CA5A/CA-VA Protein (His Tag); CA5 Protein; CA5AD Protein; CA5D Protein; Carbonic Anhydrase VA Protein; CAV Protein; CAVA Protein; GS1-21A4.1 Protein; carbonic anhydrase VA; mitochondrial; CA5A active protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH5.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala40-Ser305

• Species: Human
• Activity: Measured by its esterase activity. The specific activity is >500pmoles/min/ug.
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human CA5A (NP_001730.1)(Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA5A active protein

Background: Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A/CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

References

Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.

NCBI and Uniprot Product Information

• NCBI GI #: 4502521
• NCBI GeneID: 763
• NCBI Accession #: NP_001730.1
• NCBI GenBank Nucleotide #: NM_001739.1
• UniProt Accession #: P35218; B2RPF2
• Molecular Weight: 34,750 Da
• NCBI Official Full Name: carbonic anhydrase 5A, mitochondrial
• NCBI Official Synonym Full Names: carbonic anhydrase VA, mitochondrial
• NCBI Official Symbol: CA5A
• NCBI Official Synonym Symbols: CA5; CAV; CAVA
• NCBI Protein Information: carbonic anhydrase 5A, mitochondrial; CA-VA; carbonic dehydratase; carbonate dehydratase VA; carbonic anhydrase V, mitochondrial
• UniProt Protein Name: Carbonic anhydrase 5A, mitochondrial
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA5A
• UniProt Synonym Gene Names: CA5; CA-VA
• UniProt Entry Name: CAH5A_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq, Jul 2008]

Uniprot Description

Car5a: Reversible hydration of carbon dioxide. Low activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Mitochondrial; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process

Disease: Carbonic Anhydrase Va Deficiency, Hyperammonemia Due To