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SDS-Page

C-Reactive Protein Recombinant Protein | CRP recombinant protein

C-Reactive Protein Protein, Mouse, Recombinant (His Tag)

Purity
>94% as determined by SDS-PAGE
Synonyms
C-Reactive Protein; C-Reactive Protein Protein; Mouse; Recombinant (His Tag); Mouse CRP/C-Reactive Protein (His Tag); AI255847 Protein; C-reactive protein; pentraxin-related; CRP recombinant protein
Ordering
For Research Use Only!
Host
HEK293 Cells
Purity/Purification
>94% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser225
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu 21
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse CRP (NP_031794.3)(Met 1-Ser 225) was expressed, with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

SDS-Page

SDS-Page
Related Product Information for CRP recombinant protein
Background: C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
References
Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12. Thompson D. et al., 1999, Structure. 7(2): 169-77.

NCBI and Uniprot Product Information

Molecular Weight
The secreted recombinant mouse CRP consists of 216 amino acids and has a calculated molecular mass of 24.4kDa. As a result of glycosylation, the apparent molecular mass of rmCRP is approximately 30kDa in SDS-PAGE under reducing conditions.
Protein Family

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Product Notes

The CRP (Catalog #AAA8122726) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser22 5. It is sometimes possible for the material contained within the vial of "C-Reactive Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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