TWEAK/TNFSF12 Cell Lysate | TNFSF12 cell lysate
Mouse TWEAK/TNFSF12 HEK293 Overexpression Lysate
Gene Names
Tnfsf12; Dr3l; Apo3l; Dr3lg; Tweak
Applications
Western Blot
Synonyms
TWEAK/TNFSF12; Mouse TWEAK/TNFSF12 HEK293 Overexpression Lysate; Mouse TNFSF12 HEK293 Cell Lysate (WB positive control); tumor necrosis factor (ligand) superfamily; member 12; Mouse Apo3l Overexpression Lysate; Mouse Dr3l Overexpression Lysate; Mouse Dr3lg Overexpression Lysate; Mouse Tweak Overexpression Lysate; TNFSF12 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
405aa
Sequence
Arg105-His249
Applicable Applications for TNFSF12 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Tnfsf12 (NP_035744.1) (Arg105-His249) was expressed with the Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TNFSF12 cell lysate
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
This Mouse TWEAK/TNFSF12 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TWEAK/TNFSF12 protein from the overexpression lysate was verified.
This Mouse TWEAK/TNFSF12 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TWEAK/TNFSF12 protein from the overexpression lysate was verified.
Product Categories/Family for TNFSF12 cell lysate
References
Wiley SR, et al. (2004) TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cytokine Growth Factor Rev. 14(3-4):241-9.
Campbell S, et al. (2006) The role of TWEAK/Fn14 in the pathogenesis of inflammation and systemic autoimmunity. Front Biosci. 9:2273-84.
Lynch CN, et al. (1999) TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 274(13):8455-9.
Campbell S, et al. (2006) The role of TWEAK/Fn14 in the pathogenesis of inflammation and systemic autoimmunity. Front Biosci. 9:2273-84.
Lynch CN, et al. (1999) TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 274(13):8455-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,441 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
Dr3l; Apo3l; Dr3lg; Tweak
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
Protein Family
UniProt Gene Name
Tnfsf12
UniProt Synonym Gene Names
TWEAK
UniProt Entry Name
TNF12_MOUSE
Uniprot Description
TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines (By similarity)
Protein type: Cytokine; Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; extracellular region
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: apoptosis; multicellular organismal development; positive regulation of protein catabolic process; immune response; angiogenesis; cell differentiation
Research Articles on TNFSF12
Similar Products
Product Notes
The TNFSF12 tnfsf12 (Catalog #AAA8116437) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 405aa. AAA Biotech's TWEAK/TNFSF12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TNFSF12 tnfsf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg105-His 249. It is sometimes possible for the material contained within the vial of "TWEAK/TNFSF12, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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