Rabbit anti-Human, Mouse CSNK1E Polyclonal Antibody | anti-CSNK1E antibody

CSNK1E Antibody

Cat. Num. AAA7125033

• Gene Names: CSNK1E; HCKIE; CKIepsilon
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: CSNK1E; Polyclonal Antibody; CSNK1E Antibody; casein kinase 1; epsilon; anti-CSNK1E antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol

• Applicable Applications for anti-CSNK1E antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000-1:5000; WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Fusion protein of human CSNK1E
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MBS7125033(CSNK1E Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using MBS7125033(CSNK1E Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200))

SDS-Page

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, A431 cells, Primary antibody: MBS7125033(CSNK1E Antibody) at dilution 1/1550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Product Categories/Family for anti-CSNK1E antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4503093
• NCBI GeneID: 1454
• NCBI Accession #: NP_001885.1
• NCBI GenBank Nucleotide #: NM_001894.4
• UniProt Accession #: P49674
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Synonym Full Names: casein kinase 1, epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; CKIepsilon
• NCBI Protein Information: casein kinase I isoform epsilon; CKIe; CKI-epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; regulation of circadian rhythm; DNA repair; signal transduction; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle