ROBO4 cell lysate
Human ROBO4 HEK293 Overexpression Lysate
Gene Names
ROBO4; MRB; ECSM4
Applications
Western Blot
Synonyms
ROBO4; Human ROBO4 HEK293 Overexpression Lysate; Human ROBO4 HEK293 Cell Lysate (WB positive control); roundabout; axon guidance receptor; homolog 4 (Drosophila); Human ECSM4 Overexpression Lysate; Human MRB Overexpression Lysate; ROBO4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
453aa
Sequence
Met1-Glu469
Applicable Applications for ROBO4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ROBO4 (Q8WZ75-1) N-terminal four domains (Met 1-Glu 469) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ROBO4 cell lysate
Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.
This Human ROBO4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ROBO4 protein from the overexpression lysate was verified.
This Human ROBO4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ROBO4 protein from the overexpression lysate was verified.
References
Huminiecki L., et al., 2002, Genomics 79:547-552. Park, K.W. et al., 2003, Dev Biol. 261 (1):251-67. Yoshikawa, M. et al., 2008, Protein Expr Purif. 61 (1):78-82. Jones, C.A. et al., 2008, Nat Med. 14 (4):448-53. Koch, A.W. et al., 2011, Dev Cell. 20 (1):33-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,282 Da
NCBI Official Full Name
roundabout homolog 4 isoform 2
NCBI Official Synonym Full Names
roundabout guidance receptor 4
NCBI Official Symbol
ROBO4
NCBI Official Synonym Symbols
MRB; ECSM4
NCBI Protein Information
roundabout homolog 4
UniProt Protein Name
Roundabout homolog 4
Protein Family
UniProt Gene Name
ROBO4
UniProt Entry Name
ROBO4_HUMAN
Uniprot Description
ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: external side of plasma membrane; integral to membrane
Molecular Function: receptor activity
Biological Process: angiogenesis; cell differentiation; negative regulation of cell migration; regulation of apoptosis; regulation of cell migration
Research Articles on ROBO4
Similar Products
Product Notes
The ROBO4 robo4 (Catalog #AAA8114842) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 453aa. AAA Biotech's ROBO4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ROBO4 robo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Glu46 9. It is sometimes possible for the material contained within the vial of "ROBO4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
