Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Flow Cytometry (FC/FACS) (Figure-1: Cell surface flowcytometric analysis of CD24 in human PBMC (Lymphocytes) using 0.5 ug/10^6 Cells of Anti- CD24 antibody (Clone: ML5). Green represent isotype control and red represent Anti CD24 (MBS669234). Goat Anti-mouse PE conjugate was used as the secondary antibody.)

Mouse anti-Human CD24 Monoclonal Antibody | anti-CD24 antibody

Monoclonal Antibody to CD24 (Clone: ML5)

Gene Names
CD24; CD24A
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein G Chromatography Purified
Synonyms
CD24; Monoclonal Antibody; Monoclonal Antibody to CD24 (Clone: ML5); Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; anti-CD24 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
ML5
Purity/Purification
Protein G Chromatography Purified
Form/Format
25ug ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
80
Applicable Applications for anti-CD24 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 0.5-1 ug/10^6Cells
IHC: 5-10 ug/ml
Immunogen
CD24
Immunogen Information
Full length CD24 protein was used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months. For long-term storage; store at -20 degree C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (FC/FACS)

(Figure-1: Cell surface flowcytometric analysis of CD24 in human PBMC (Lymphocytes) using 0.5 ug/10^6 Cells of Anti- CD24 antibody (Clone: ML5). Green represent isotype control and red represent Anti CD24 (MBS669234). Goat Anti-mouse PE conjugate was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Figure-1: Cell surface flowcytometric analysis of CD24 in human PBMC (Lymphocytes) using 0.5 ug/10^6 Cells of Anti- CD24 antibody (Clone: ML5). Green represent isotype control and red represent Anti CD24 (MBS669234). Goat Anti-mouse PE conjugate was used as the secondary antibody.)

Immunohistochemistry

(Figure-2: Immunohistochemical analysis of CD24 in Human Tonsil tissue using Anti-CD24 antibody (Clone: ML5) at 5 µg/ml.)

Immunohistochemistry (Figure-2: Immunohistochemical analysis of CD24 in Human Tonsil tissue using Anti-CD24 antibody (Clone: ML5) at 5 µg/ml.)
Related Product Information for anti-CD24 antibody
The antigen is CD24 with a molecular weight of 35 - 45kD. The epitope has not been further characterized. Antigen distribution: Isolated cells: granulocytes. Tissue sections: B-cells (germinal centers weak); follicular dendritic cells; variety of epithelial cell types
Product Categories/Family for anti-CD24 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35-45kD
NCBI Official Full Name
signal transducer CD24 isoform a preproprotein
NCBI Official Synonym Full Names
CD24 molecule
NCBI Official Symbol
CD24
NCBI Official Synonym Symbols
CD24A
NCBI Protein Information
signal transducer CD24
UniProt Protein Name
Signal transducer CD24
Protein Family
UniProt Gene Name
CD24
UniProt Synonym Gene Names
CD24A

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed:11313396). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity ().

Research Articles on CD24

Similar Products

Product Notes

The CD24 cd24 (Catalog #AAA669234) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD24 (Clone: ML5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD24 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). FC/FACS: 0.5-1 ug/10^6Cells IHC: 5-10 ug/ml. Researchers should empirically determine the suitability of the CD24 cd24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD24, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.