Phospholipase A2 IIE/PLA2G2E Cell Lysate | PLA2G2E cell lysate
Mouse Phospholipase A2 IIE/PLA2G2E HEK293 Overexpression Lysate
Applications
Western Blot
Synonyms
Phospholipase A2 IIE/PLA2G2E; Mouse Phospholipase A2 IIE/PLA2G2E HEK293 Overexpression Lysate; Mouse PLA2G2E HEK293 Cell Lysate (WB positive control); phospholipase A2; group IIE; PLA2G2E cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
134aa
Sequence
Met1-Cys142
Applicable Applications for PLA2G2E cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse PLA2G2E (Q9QUL3) (Met 1-Cys 142) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PLA2G2E cell lysate
Group IIE secretory phospholipase A2, also known as GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E and PLA2G2E is a secreted protein that belongs to the phospholipase A2 family. Mammalian secretory phospholipase A2s (sPLA2s) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2/PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2/PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2/PLA2. Due to the increased presence and activity of Phospholipases A2/PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site. PLA2G2E catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
This Mouse Phospholipase A2 IIE/PLA2G2E overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Phospholipase A2 IIE/PLA2G2E protein from the overexpression lysate was verified.
This Mouse Phospholipase A2 IIE/PLA2G2E overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Phospholipase A2 IIE/PLA2G2E protein from the overexpression lysate was verified.
References
Dennis EA. et al., 1994, J. Biol. Chem. 269 (18): 13057-60. Nicolas JP, et al., 1997, J. Biol. Chem. 272 (11): 7173-81. Six DA, et al., 2000, Biochim. Biophys. Acta 1488 (1-2): 1-19. Suzuki N., et al., 2000, J. Biol. Chem. 275:5785-93.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,943 Da
NCBI Official Full Name
group IIE secretory phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IIE
NCBI Official Symbol
Pla2g2e
NCBI Protein Information
group IIE secretory phospholipase A2; sPLA2-IIE; GIIE sPLA2; mGIIEsPLA2s; phosphatidylcholine 2-acylhydrolase 2E
UniProt Protein Name
Group IIE secretory phospholipase A2
Protein Family
UniProt Gene Name
Pla2g2e
UniProt Synonym Gene Names
GIIE sPLA2; sPLA2-IIE
UniProt Entry Name
PA2GE_MOUSE
Uniprot Description
PLA2G2E: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids. Belongs to the phospholipase A2 family.
Protein type: Lipid Metabolism - ether lipid; Phospholipase; Secreted; Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid
Cellular Component: extracellular region
Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding
Biological Process: phospholipid metabolic process; lipid metabolic process; lipid catabolic process
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Product Notes
The PLA2G2E pla2g2e (Catalog #AAA8116786) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 134aa. AAA Biotech's Phospholipase A2 IIE/PLA2G2E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PLA2G2E pla2g2e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Cys14 2. It is sometimes possible for the material contained within the vial of "Phospholipase A2 IIE/PLA2G2E, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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