Typical Testing Data/Standard Curve (for reference only)
Mouse Group 2E secretory phospholipase A2, PLA2G2E ELISA Kit | PLA2G2E elisa kit
Mouse Group 2E secretory phospholipase A2, PLA2G2E ELISA Kit
Gene Names
PLA2G2E; sPLA2-IIE; GIIE sPLA2
Reactivity
Mouse
Synonyms
Group 2E secretory phospholipase A2; PLA2G2E; Mouse Group 2E secretory phospholipase A2; PLA2G2E ELISA Kit; PLA2G2E elisa kit
Reactivity
Mouse
Sequence Length
142
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
20-6000ng/L
Sensitivity
11.12ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G2E elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Group 2E secretory phospholipase A2 (also known as PLA2G2E) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PLA2G2E antibody. PLA2G2E present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PLA2G2E Antibody is added and binds to PLA2G2E in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G2E antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G2E. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PLA2G2E antibody. PLA2G2E present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PLA2G2E Antibody is added and binds to PLA2G2E in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G2E antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G2E. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
group IIE secretory phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2 group IIE
NCBI Official Symbol
PLA2G2E
NCBI Official Synonym Symbols
sPLA2-IIE; GIIE sPLA2
NCBI Protein Information
group IIE secretory phospholipase A2
UniProt Protein Name
Group IIE secretory phospholipase A2
Protein Family
UniProt Gene Name
PLA2G2E
UniProt Synonym Gene Names
GIIE sPLA2; sPLA2-IIE
UniProt Entry Name
PA2GE_HUMAN
Uniprot Description
PLA2G2E: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids. Belongs to the phospholipase A2 family.
Protein type: Phospholipase; Secreted, signal peptide; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Secreted
Chromosomal Location of Human Ortholog: 1p36.13
Cellular Component: extracellular region
Molecular Function: phospholipase A2 activity; calcium ion binding
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; inflammatory response; lipid catabolic process
Research Articles on PLA2G2E
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Product Notes
The Mouse PLA2G2E pla2g2e (Catalog #AAA1605006) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1605006 ELISA Kit recognizes Mouse PLA2G2E. It is sometimes possible for the material contained within the vial of "Group 2E secretory phospholipase A2, PLA2G2E, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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