Western Blot (WB)
(Anti-Pla2g12b rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse liver tissue lysateLane B: HT1080 Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)
Rabbit anti-Mouse PLA2G12B Polyclonal Antibody | anti-PLA2G12B antibody
Anti-PLA2G12B Antibody, Rabbit Polyclonal
Gene Names
Pla2g12b; Fksg71; hlb218; Pla2g13; 2010002E04Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PLA2G12B; Polyclonal Antibody; Anti-PLA2G12B Antibody; Rabbit Polyclonal; PLA2G12B Antibody; Rabbit PAb; Antigen Affinity Purified; phospholipase A2; group XIIB; Anti-2010002E04Rik Antibody; Anti-Fksg71 Antibody; Anti-hlb218 Antibody; Anti-Pla2g13 Antibody; anti-PLA2G12B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse PLA2G12B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PLA2G12B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the N-terminus of the Mouse PLA2G12B
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse PLA2G12B, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Pla2g12b rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse liver tissue lysateLane B: HT1080 Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)
Western Blot (WB)
(Anti-Pla2g12b rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse liver tissue lysateLane B: HT1080 Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)
Related Product Information for anti-PLA2G12B antibody
Group XIIB secretory phospholipase A2-like protein, also known as Group XIII secretory phospholipase A2-like protein, GXIII sPLA2-like, sPLA2-GXIIB, GXIIB, PLA2G13 and PLA2G12B, is a secreted protein that belongs to the phospholipase A2 family. PLA2G12B/PLA2G13 is strongly expressed in liver, small intestine and kidney. Mammalian secretory phospholipase A2s (sPLA2s) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2/PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2/PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2/PLA2. Due to the increased presence and activity of Phospholipases A2/PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site.
References
Dennis EA. et al., 1994, J. Biol. Chem. 269 (18): 13057-60. Nicolas JP, et al., 1997, J. Biol. Chem. 272 (11): 7173-81. Six DA, et al., 2000, Biochim. Biophys. Acta 1488 (1-2): 1-19. Mounier, C.M. et al., 2008, Br J Cancer. 98 (3):587-95.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,736 Da
NCBI Official Full Name
Group XIIB secretory phospholipase A2-like protein
NCBI Official Synonym Full Names
phospholipase A2, group XIIB
NCBI Official Symbol
Pla2g12b
NCBI Official Synonym Symbols
Fksg71; hlb218; Pla2g13; 2010002E04Rik
NCBI Protein Information
group XIIB secretory phospholipase A2-like protein; GXIIB; sPLA2-GXIIB; group XIIB phospholipase A2; phospholipase A2, group XIII; group XIII secretory phospholipase A2-like protein
UniProt Protein Name
Group XIIB secretory phospholipase A2-like protein
Protein Family
UniProt Gene Name
Pla2g12b
UniProt Synonym Gene Names
Pla2g13; Fksg71; GXIII sPLA2-like; GXIIB
UniProt Entry Name
PG12B_MOUSE
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Product Notes
The PLA2G12B pla2g12b (Catalog #AAA8103399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLA2G12B Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G12B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PLA2G12B pla2g12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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