GASP-1/WFIKKN2 Cell Lysate | WFIKKN2 cell lysate
Human GASP-1/WFIKKN2 HEK293 Overexpression Lysate
Gene Names
WFIKKN2; GASP-1; WFDC20B; hGASP-1; WFIKKNRP
Applications
Western Blot
Synonyms
GASP-1/WFIKKN2; Human GASP-1/WFIKKN2 HEK293 Overexpression Lysate; Human WFIKKN2/GASP-1 HEK293 Cell Lysate (WB positive control); WAP; follistatin/kazal; immunoglobulin; kunitz and netrin domain containing 2; Human GASP-1 Overexpression Lysate; Human hGASP-1 Overexpression Lysate; Human WFDC20B Overexpression Lysate; Human WFIKKNRP Overexpression Lysate; WFIKKN2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
553aa
Sequence
Met1-His576
Applicable Applications for WFIKKN2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human WFIKKN2 (NP_783165.1) extracellular domain (Met 1-His 576) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for WFIKKN2 cell lysate
WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2, also known as Growth and differentiation factor-associated serum protein 1, WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-related protein, WFIKKN-related protein, WFIKKN2 and GASP1, is a secreted protein that belongs to the WFIKKN family. WFIKKN2 contains two BPTI/Kunitz inhibitor domains, one Ig-like C2-type (immunoglobulin-like) domain, one Kazal-like domain, one NTR domain and one WAP domain. WFIKKN2 is primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney. WFIKKN2 is a protease-inhibitor that contains multiple distinct protease inhibitor domains. It probably has serine protease-and metalloprotease-inhibitor activity. It inhibits the biological activity of mature myostatin, but not activin. WFIKKN2 protein binds mature GDF8/myostatin and myostatin propeptide and inhibits the biological activity of myostatin.
This Human GASP-1/WFIKKN2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GASP-1/WFIKKN2 protein from the overexpression lysate was verified.
This Human GASP-1/WFIKKN2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GASP-1/WFIKKN2 protein from the overexpression lysate was verified.
Product Categories/Family for WFIKKN2 cell lysate
References
Clark H.F.et al., 2003, Genome Res. 13:2265-70. Hill J.J.et al., 2003, Mol. Endocrinol. 17:1144-54. Kondas, K. et al., 2008, J Biol Chem 283 (35):23677-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,941 Da
NCBI Official Full Name
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
NCBI Official Synonym Full Names
WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
NCBI Official Symbol
WFIKKN2
NCBI Official Synonym Symbols
GASP-1; WFDC20B; hGASP-1; WFIKKNRP
NCBI Protein Information
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2; WFIKKN-related protein; WAP four-disulfide core domain 20B; multivalent protease inhibitor protein; WAP, FS, Ig, two KU and NTR module related protein; growth and differentiation fact
UniProt Protein Name
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
UniProt Gene Name
WFIKKN2
UniProt Synonym Gene Names
GASP1; WFIKKNRP; GASP-1; hGASP-1
UniProt Entry Name
WFKN2_HUMAN
NCBI Description
The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases. [provided by RefSeq, Jul 2008]
Uniprot Description
WFIKKN2: Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin. Belongs to the WFIKKN family.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity
Biological Process: transforming growth factor beta receptor signaling pathway; palate development; muscle fiber development; negative regulation of protein binding; skeletal development; negative regulation of DNA binding
Research Articles on WFIKKN2
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Product Notes
The WFIKKN2 wfikkn2 (Catalog #AAA8114866) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 553aa. AAA Biotech's GASP-1/WFIKKN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the WFIKKN2 wfikkn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-His57 6. It is sometimes possible for the material contained within the vial of "GASP-1/WFIKKN2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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