GASP-1/WFIKKN2 Recombinant Protein | WFIKKN2 recombinant protein

GASP-1/WFIKKN2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121048

• Gene Names: WFIKKN2; GASP-1; WFDC20B; hGASP-1; WFIKKNRP
• Purity: >96% as determined by SDS-PAGE
• Synonyms: GASP-1/WFIKKN2; GASP-1/WFIKKN2 Protein; Human; Recombinant (His Tag); Human WFIKKN2/GASP-1 Protein (His Tag); GASP-1 Protein; hGASP-1 Protein; WFDC20B Protein; WFIKKNRP Protein; WAP; follistatin/kazal; immunoglobulin; kunitz and netrin domain containing 2; WFIKKN2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His576

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human WFIKKN2 (NP_783165.1) extracellular domain (Met 1-His 576) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for WFIKKN2 recombinant protein

Background: WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2, also known as Growth and differentiation factor-associated serum protein 1, WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-related protein, WFIKKN-related protein, WFIKKN2 and GASP1, is a secreted protein that belongs to the WFIKKN family. WFIKKN2 contains two BPTI/Kunitz inhibitor domains, one Ig-like C2-type (immunoglobulin-like) domain, one Kazal-like domain, one NTR domain and one WAP domain. WFIKKN2 is primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney. WFIKKN2 is a protease-inhibitor that contains multiple distinct protease inhibitor domains. It probably has serine protease- and metalloprotease-inhibitor activity. It inhibits the biological activity of mature myostatin, but not activin. WFIKKN2 protein binds mature GDF8/myostatin and myostatin propeptide and inhibits the biological activity of myostatin.

Product Categories/Family for WFIKKN2 recombinant protein

Serine Proteases and Regulators

References

Clark H.F.et al., 2003,Genome Res. 13:2265-70. Hill J.J.et al., 2003, Mol. Endocrinol. 17:1144-54. Konda¡s,K. et al., 2008, J Biol Chem 283 (35):23677-84.

NCBI and Uniprot Product Information

• NCBI GI #: 28212222
• NCBI GeneID: 124857
• NCBI Accession #: NP_783165.1
• NCBI GenBank Nucleotide #: NM_175575.5
• UniProt Accession #: Q8TEU8; Q6UXZ9
• Molecular Weight: 63,941 Da
• NCBI Official Full Name: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
• NCBI Official Synonym Full Names: WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
• NCBI Official Symbol: WFIKKN2
• NCBI Official Synonym Symbols: GASP-1; WFDC20B; hGASP-1; WFIKKNRP
• NCBI Protein Information: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2; WFIKKN-related protein; WAP four-disulfide core domain 20B; multivalent protease inhibitor protein; WAP, FS, Ig, two KU and NTR module related protein; growth and differentiation fact
• UniProt Protein Name: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
• Protein Family: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein
• UniProt Gene Name: WFIKKN2
• UniProt Synonym Gene Names: GASP1; WFIKKNRP; GASP-1; hGASP-1
• UniProt Entry Name: WFKN2_HUMAN

NCBI Description

The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases. [provided by RefSeq, Jul 2008]

Uniprot Description

WFIKKN2: Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin. Belongs to the WFIKKN family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity

Biological Process: transforming growth factor beta receptor signaling pathway; palate development; muscle fiber development; negative regulation of protein binding; skeletal development; negative regulation of DNA binding