EDEM2 cell lysate
Human EDEM2 HEK293 Overexpression Lysate
Gene Names
EDEM2; C20orf31; C20orf49; bA4204.1
Applications
Western Blot
Synonyms
EDEM2; Human EDEM2 HEK293 Overexpression Lysate; Human EDEM2/C20orf31 HEK293 Cell Lysate (WB positive control); ER degradation enhancer; mannosidase alpha-like 2; Human bA4204.1 Overexpression Lysate; Human C20orf31 Overexpression Lysate; Human C20orf49 Overexpression Lysate; Human UNQ573/PRO1135 Overexpression Lysate; EDEM2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
482aa
Sequence
Met1-Lys492
Applicable Applications for EDEM2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human EDEM2 (AAH01371.1) (Met1-Lys492) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for EDEM2 cell lysate
EDEM2, also known as C2orf31, belongs to a family of proteins involved in ER-associated degradation (ERAD) of glycoproteins. In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome. Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules from the folding pathway, and facilitates their targeting for disposal. In humans there are a total of three EDEM homologs. The amino acid sequences of these proteins are different from other lectins but are closely related to the Class I mannosidases (family 47 glycosidases). EDEM2 is one of the EDEM homologs. Overexpression of EDEM2 accelerates the degradation of misfolded alpha1-antitrypsin, indicating that the protein is involved in ERAD.
This Human EDEM2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EDEM2 protein from the overexpression lysate was verified.
This Human EDEM2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EDEM2 protein from the overexpression lysate was verified.
References
Mast SW. et al., 2005, Glycobiology. 15 (4): 421-36. Olivari S. et al., 2005, J Biol Chem. 280 (4): 2424-8. Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,319 Da
NCBI Official Full Name
ER degradation enhancer, mannosidase alpha-like 2
NCBI Official Synonym Full Names
ER degradation enhancing alpha-mannosidase like protein 2
NCBI Official Symbol
EDEM2
NCBI Official Synonym Symbols
C20orf31; C20orf49; bA4204.1
NCBI Protein Information
ER degradation-enhancing alpha-mannosidase-like protein 2
Protein Family
NCBI Description
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM, Mar 2008]
Research Articles on EDEM2
Similar Products
Product Notes
The EDEM2 (Catalog #AAA8115551) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 482aa. AAA Biotech's EDEM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the EDEM2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys49 2. It is sometimes possible for the material contained within the vial of "EDEM2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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