ESM1 recombinant protein

ESM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120861

• Gene Names: ESM1; endocan
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ESM1; ESM1 Protein; Human; Recombinant (His Tag); Human ESM1/Endocan Protein (His Tag); endocan Protein; endothelial cell-specific molecule 1; ESM1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg184

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ESM1 (NP_008967.1)(Met1-Arg184) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ESM1 recombinant protein

Background: ESM1 is a secreted protein which is produced by adipocytes. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. ESM1 is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Recently, ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.

References

Cong R, et al. (2006) Hhex is a direct repressor of endothelial cell-specific molecule 1 (ESM-1). Biochem. Biophys Res Commun. 346(2):535-45.Aitkenhead M, et al. (2002) Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: induction of ESM-1, (beta)ig-h3, and NrCAM. Microvasc Res. 63(2): 159-71.Lassalle P, et al. (1996) ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J Biol Chem. 271(34):20458-64.

NCBI and Uniprot Product Information

• NCBI GI #: 5901946
• NCBI GeneID: 11082
• NCBI Accession #: NP_008967.1
• NCBI GenBank Nucleotide #: NM_007036.4
• UniProt Accession #: Q9NQ30; Q15330; Q3V4E3; Q96ES3; B2R4G3
• Molecular Weight: 20,095 Da
• NCBI Official Full Name: endothelial cell-specific molecule 1 isoform a
• NCBI Official Synonym Full Names: endothelial cell-specific molecule 1
• NCBI Official Symbol: ESM1
• NCBI Official Synonym Symbols: endocan
• NCBI Protein Information: endothelial cell-specific molecule 1; ESM-1
• UniProt Protein Name: Endothelial cell-specific molecule 1
• Protein Family: Endothelial cell-specific molecule
• UniProt Gene Name: ESM1
• UniProt Synonym Gene Names: ESM-1
• UniProt Entry Name: ESM1_HUMAN

Uniprot Description

ESM1: May have potent implications in lung endothelial cell- leukocyte interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: integrin binding; insulin-like growth factor binding; hepatocyte growth factor receptor binding

Biological Process: positive regulation of cell proliferation; regulation of cell growth; angiogenesis; sprouting angiogenesis