Western Blot (WB)
(Anti-IRX4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:58 kDa (We are unsure as to the identity of these extra bands.))
Rabbit IRX4 Polyclonal Antibody | anti-IRX4 antibody
Anti-IRX4 Antibody, Rabbit Polyclonal
Gene Names
IRX4; IRXA3; MGC131996
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
IRX4; Polyclonal Antibody; Anti-IRX4 Antibody; Rabbit Polyclonal; IRX4 Antibody; Rabbit PAb; Antigen Affinity Purified; iroquois homeobox 4; Anti-IRXA3 Antibody; anti-IRX4 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human IRX4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-IRX4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
A synthetic peptide corresponding to the center region of the Human IRX4
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human IRX4, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-IRX4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:58 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-IRX4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:58 kDa (We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of IRX4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human IRX4 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of IRX4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human IRX4 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54.45kD.
Jurkat cell line: weak bands at 24 and 40kD.
Jurkat cell line: weak bands at 24 and 40kD.
NCBI Official Full Name
iroquois-class homeodomain protein IRX-4
NCBI Official Synonym Full Names
iroquois homeobox 4
NCBI Official Symbol
IRX4
NCBI Official Synonym Symbols
IRXA3; MGC131996
NCBI Protein Information
iroquois-class homeodomain protein IRX-4; OTTHUMP00000115477; OTTHUMP00000220473; OTTHUMP00000220474; OTTHUMP00000220475; OTTHUMP00000220476; homeodomain protein IRXA3; iroquois homeobox protein 4
UniProt Protein Name
Iroquois-class homeodomain protein IRX-4
Protein Family
UniProt Gene Name
IRX4
UniProt Synonym Gene Names
IRXA3
UniProt Entry Name
IRX4_HUMAN
Uniprot Description
IRX4: Likely to be an important mediator of ventricular differentiation during cardiac development. Belongs to the TALE/IRO homeobox family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5p15.3
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding
Biological Process: regulation of transcription, DNA-dependent; heart development; establishment of organ orientation
Research Articles on IRX4
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Product Notes
The IRX4 irx4 (Catalog #AAA8102705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRX4 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's IRX4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the IRX4 irx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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