EDEM2 / C20orf31 recombinant protein

Recombinant Human EDEM2 / C20orf31 Protein (His tag)

Cat. Num. AAA2545215

• Gene Names: EDEM2; C20orf31; C20orf49; bA4204.1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: EDEM2 / C20orf31; Recombinant Human EDEM2 / C20orf31 Protein (His tag); EDEM2; C20orf31; C20orf49; EDEM2 / C20orf31 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 578

• Application Notes: The recombinant human EDEM2 consists of 482 amino acids and predicts a molecular mass of 54 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for EDEM2 / C20orf31 recombinant protein

Background: EDEM2, also known as C20orf31, belongs to a family of proteins involved in ER-associated degradation (ERAD) of glycoproteins. In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome. Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules from the folding pathway, and facilitates their targeting for disposal. In humans there are a total of three EDEM homologs. The amino acid sequences of these proteins are different from other lectins but are closely related to the Class I mannosidases (family 47 glycosidases). EDEM2 is one of the EDEM homologs. Overexpression of EDEM2 accelerates the degradation of misfolded alpha1-antitrypsin, indicating that the protein is involved in ERAD.

Description: A DNA sequence encoding the human EDEM2 (AAH01371.1) (Met1-Lys492) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 12655043
• NCBI GeneID: 55741
• Molecular Weight: 60,319 Da
• NCBI Official Full Name: ER degradation enhancer, mannosidase alpha-like 2
• NCBI Official Synonym Full Names: ER degradation enhancing alpha-mannosidase like protein 2
• NCBI Official Symbol: EDEM2
• NCBI Official Synonym Symbols: C20orf31; C20orf49; bA4204.1
• NCBI Protein Information: ER degradation-enhancing alpha-mannosidase-like protein 2
• UniProt Protein Name: ER degradation-enhancing alpha-mannosidase-like protein 2
• UniProt Gene Name: EDEM2
• UniProt Synonym Gene Names: C20orf31; C20orf49

NCBI Description

In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM, Mar 2008]

Uniprot Description

EDEM2: Involved in endoplasmic reticulum-associated degradation (ERAD) that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It lacks mannosidase activity. Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. Belongs to the glycosyl hydrolase 47 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: endoplasmic reticulum

Molecular Function: alpha-mannosidase activity; mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

Biological Process: ER-associated protein catabolic process; N-glycan processing; unfolded protein response