EDAR cell lysate

Human EDAR HEK293 Overexpression Lysate

Cat. Num. AAA8114590

• Gene Names: EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
• Applications: Western Blot
• Synonyms: EDAR; Human EDAR HEK293 Overexpression Lysate; Human EDAR/DL HEK293 Cell Lysate (WB positive control); ectodysplasin A receptor; Human DL Overexpression Lysate; Human ECTD10A Overexpression Lysate; Human ECTD10B Overexpression Lysate; Human ED1R Overexpression Lysate; Human ED3 Overexpression Lysate; Human ED5 Overexpression Lysate; Human EDA-A1R Overexpression Lysate; Human EDA1R Overexpression Lysate; Human EDA3 Overexpression Lysate; Human HRM1 Overexpression Lysate; EDAR cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 404aa
• Sequence: Met1-Ile189

Applicable Applications for EDAR cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human EDAR (NP_071731.1) extracellular domain (Met 1-Ile 189) was fused with the Fc region of human IgG1 at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.