CSF3R/G-CSFR Cell Lysate | CSF3R cell lysate
Human CSF3R/G-CSFR HEK293 Overexpression Lysate
Gene Names
CSF3R; CD114; GCSFR
Applications
Western Blot
Synonyms
CSF3R/G-CSFR; Human CSF3R/G-CSFR HEK293 Overexpression Lysate; Human G-CSFR/CD114/CSF3R HEK293 Cell Lysate (WB positive control); colony stimulating factor 3 receptor (granulocyte); Human CD114 Overexpression Lysate; Human CSF3R Overexpression Lysate; Human G-CSF R Overexpression Lysate; Human GCSFR Overexpression Lysate; CSF3R cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
603aa
Sequence
Met1-Pro621
Applicable Applications for CSF3R cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
The mature form of human G-CSF receptor (NP_000751.1) extracellular domain (Met 1-Pro 621) with five aminio acids at the C-terminus was expressed and purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CSF3R cell lysate
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.
This Human CSF3R/G-CSFR overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CSF3R/G-CSFR protein from the overexpression lysate was verified.
This Human CSF3R/G-CSFR overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CSF3R/G-CSFR protein from the overexpression lysate was verified.
Product Categories/Family for CSF3R cell lysate
References
Kasper B, et al. (1999) Association of src-kinase Lyn and non-src-kinase Syk with the granulocyte colony-stimulating factor receptor (G-CSFR) is not abrogated in neutrophils from severe congenital neutropenia patients with point mutations in the G-CSFR mRNA. Int J Hematol. 70(4): 241-7.Hollenstein U, et al. (2000) Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils. J Infect Dis. 182(1): 343-6.Kindwall-Keller TL, et al. (2008) Role of the proteasome in modulating native G-CSFR expression. Cytokine. 43(2): 114-23.Beel K, et al. (2009) G-CSF receptor (CSF3R) mutations in X-linked neutropenia evolving to acute myeloid leukemia or myelodysplasia. Haematologica. 94(10): 1449-52.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,609 Da
NCBI Official Full Name
granulocyte colony-stimulating factor receptor isoform a
NCBI Official Synonym Full Names
colony stimulating factor 3 receptor
NCBI Official Symbol
CSF3R
NCBI Official Synonym Symbols
CD114; GCSFR
NCBI Protein Information
granulocyte colony-stimulating factor receptor
UniProt Protein Name
Granulocyte colony-stimulating factor receptor
Protein Family
UniProt Gene Name
CSF3R
UniProt Synonym Gene Names
GCSFR; G-CSF receptor; G-CSF-R
UniProt Entry Name
CSF3R_HUMAN
NCBI Description
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]
Uniprot Description
GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p35-p34.3
Cellular Component: integral to plasma membrane; extracellular region
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity
Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; signal transduction; cell adhesion
Disease: Neutrophilia, Hereditary
Research Articles on CSF3R
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Product Notes
The CSF3R csf3r (Catalog #AAA8113623) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 603aa. AAA Biotech's CSF3R/G-CSFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CSF3R csf3r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro62 1. It is sometimes possible for the material contained within the vial of "CSF3R/G-CSFR, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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