BAFFR/TNFRSF13C Cell Lysate | TNFRSF13C cell lysate
Mouse BAFFR/TNFRSF13C HEK293 Overexpression Lysate
Gene Names
Tnfrsf13c; Bcmd; Baffr; Bcmd1; BAFF-R; Bcmd-1; Lvis22; 2010006P15Rik
Applications
Western Blot
Synonyms
BAFFR/TNFRSF13C; Mouse BAFFR/TNFRSF13C HEK293 Overexpression Lysate; Mouse BAFFR/TNFRSF13C/CD268 HEK293 Cell Lysate (WB positive control); tumor necrosis factor receptor superfamily; member 13C; Mouse 2010006P15Rik Overexpression Lysate; Mouse BAFF-R Overexpression Lysate; Mouse Baffr Overexpression Lysate; Mouse Bcmd Overexpression Lysate; Mouse Bcmd-1 Overexpression Lysate; Mouse Bcmd1 Overexpression Lysate; Mouse Lvis22 Overexpression Lysate; TNFRSF13C cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
303aa
Sequence
Met1-Ala71
Applicable Applications for TNFRSF13C cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse BAFFR (Q9D8D0-1) extracellular domain (Met 1-Ala 71) was fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TNFRSF13C cell lysate
Tumor necrosis factor receptor superfamily, member 13C (TNFRSF13C) also known as B-cell-activating factor receptor (BAFFR) and CD268 antigen, is a member of the tumor necrosis factor receptor superfamily. A tumor necrosis factor receptor (TNFR), or death receptor, is a trimeric cytokine receptor that binds tumor necrosis factors (TNF). The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. It has been proposed that abnormally high levels of BAFFR/TNFRSF13C (CD268) may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.
This Mouse BAFFR/TNFRSF13C overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BAFFR/TNFRSF13C protein from the overexpression lysate was verified.
This Mouse BAFFR/TNFRSF13C overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BAFFR/TNFRSF13C protein from the overexpression lysate was verified.
References
Ashkenazi A, et al. (1998) Death receptors: signaling and modulation. Science. 281(5381): 1305-8.Losi CG, et al. (2005) Mutational analysis of human BAFF receptor TNFRSF13C (BAFF-R) in patients with common variable immunodeficiency. J Clin Immunol. 25(5): 496-502.Hentges KE, et al. (2002) Tnfrsf13c (Baffr) is mis-expressed in tumors with murine leukemia virus insertions at Lvis22. Genomics. 80(2): 204-12.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,576 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 13C
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 13c
NCBI Official Symbol
Tnfrsf13c
NCBI Official Synonym Symbols
Bcmd; Baffr; Bcmd1; BAFF-R; Bcmd-1; Lvis22; 2010006P15Rik
NCBI Protein Information
tumor necrosis factor receptor superfamily member 13C; BAFF receptor; BLyS receptor 3; B-cell maturation defect 1; B-cell-activating factor receptor; b cell-activating factor receptor
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 13C
Protein Family
UniProt Gene Name
Tnfrsf13c
UniProt Synonym Gene Names
Baffr; Bcmd; Br3; BAFF-R
UniProt Entry Name
TR13C_MOUSE
Uniprot Description
BAFF-R: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4); also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Cell cycle regulation; Membrane protein, integral; Cell surface
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Biological Process: B cell costimulation; regulation of immune response; B cell homeostasis; immune system process; T cell costimulation; positive regulation of B cell proliferation; positive regulation of germinal center formation; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process
Research Articles on TNFRSF13C
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Product Notes
The TNFRSF13C tnfrsf13c (Catalog #AAA8116561) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 303aa. AAA Biotech's BAFFR/TNFRSF13C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TNFRSF13C tnfrsf13c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala71. It is sometimes possible for the material contained within the vial of "BAFFR/TNFRSF13C, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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