ARMET/MANF Cell Lysate | MANF cell lysate
Mouse ARMET/MANF HEK293 Overexpression Lysate
Gene Names
Manf; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
Applications
Western Blot
Synonyms
ARMET/MANF; Mouse ARMET/MANF HEK293 Overexpression Lysate; Mouse MANF/ARMET HEK293 Cell Lysate (WB positive control); mesencephalic astrocyte-derived neurotrophic factor; Mouse 3230402M22Rik Overexpression Lysate; Mouse AA407711 Overexpression Lysate; Mouse AA408789 Overexpression Lysate; Mouse AA673178 Overexpression Lysate; Mouse Armet Overexpression Lysate; Mouse D17914 Overexpression Lysate; Mouse D18Mgi17 Overexpression Lysate; MANF cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
169aa
Sequence
Met1-Leu179
Applicable Applications for MANF cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MANF cell lysate
Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF, and ARMET, is a secreted protein that belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral midbrain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus, and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggests that it has important functions both under normal and pathological conditions in many tissue types.
This Mouse ARMET/MANF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ARMET/MANF protein from the overexpression lysate was verified.
This Mouse ARMET/MANF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ARMET/MANF protein from the overexpression lysate was verified.
References
Shridhar V., et al., 1996, Oncogene 12:1931-1939. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Petrova P., et al., 2003, J. Mol. Neurosci. 20:173-188. Lindholm, P. et al., 2008, Mol Cell Neurosci. 39 (3):356-71. Airavaara, M. et al., 2010, Exp Neurol. 225 (1):104-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,374 Da
NCBI Official Full Name
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Synonym Full Names
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Symbol
Manf
NCBI Official Synonym Symbols
Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
NCBI Protein Information
mesencephalic astrocyte-derived neurotrophic factor
Protein Family
