APCDD1 cell lysate
Human APCDD1 HEK293 Overexpression Lysate
Gene Names
APCDD1; HHS; HTS; B7323; HYPT1; DRAPC1; FP7019
Applications
Western Blot
Synonyms
APCDD1; Human APCDD1 HEK293 Overexpression Lysate; Human APCDD1 HEK293 Cell Lysate (WB positive control); adenomatosis polyposis coli down-regulated 1; Human B7323 Overexpression Lysate; Human DRAPC1 Overexpression Lysate; Human FP7019 Overexpression Lysate; Human HHS Overexpression Lysate; Human HTS Overexpression Lysate; Human HYPT1 Overexpression Lysate; APCDD1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
701aa
Sequence
Met1-Gly486
Applicable Applications for APCDD1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human APCDD1 (NP_694545.1) (Met1-Gly486) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for APCDD1 cell lysate
Osteosarcoma (OS) is the most common type of bone tumor in children and adults. The expression of APCDD1, a Wnt antagonist, was reduced in OS tissues and cells compared to adjacent normal tissue and osteoblast cells, respectively. Mechanistically, this was due to increased levels of methylation in the promoter region of the APCDD1 gene. Consistently, the DNA methyltransferase inhibitor 5-AZA-dC, reduced DNA methylation in the APCDD1 promoter, and restored APCDD1 expression in OS tissue and cells. Moreover, DNMT3a, but not DNMT1 or DNMT3b, was the major DNA methyltransferase that facilitated hyper-methylation of DNA in the APCDD1 promoter, thus reducing APCDD1 mRNA levels in OS tissues. Importantly, ectopic expression of APCDD1 suppressed activity of the Wnt/beta-Catenin signaling pathway in OS cells and inhibited their invasion and reversed their EMT-like properties, while depletion of APCDD1 promoted invasion and metastasis of osteosarcoma cells in vitro and in vivo. That APCDD1 modulates the gene expression of Wnt-and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE. In hair follicle cells APCDD1 inhibits the canonical WNT/beta-Catenin pathway and its inactivation is associated with an autosomal dominant form of hair loss. APCDD1 sustains the expression and activation of beta-Catenin.
This Human APCDD1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of APCDD1 protein from the overexpression lysate was verified.
This Human APCDD1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of APCDD1 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,797 Da
NCBI Official Full Name
protein APCDD1
NCBI Official Synonym Full Names
adenomatosis polyposis coli down-regulated 1
NCBI Official Symbol
APCDD1
NCBI Official Synonym Symbols
HHS; HTS; B7323; HYPT1; DRAPC1; FP7019
NCBI Protein Information
protein APCDD1; hypoptrichosis simplex; adenomatosis polyposis coli down-regulated 1 protein
UniProt Protein Name
Protein APCDD1
Protein Family
UniProt Gene Name
APCDD1
UniProt Synonym Gene Names
DRAPC1; FP7019
UniProt Entry Name
APCD1_HUMAN
NCBI Description
This locus encodes an inhibitor of the Wnt signaling pathway. Mutations at this locus have been associated with hereditary hypotrichosis simplex. Increased expression of this gene may also be associated with colorectal carcinogenesis.[provided by RefSeq, Sep 2010]
Uniprot Description
APCDD1: a single-pass type I membrane protein. Probably plays a role in colorectal tumorigenesis. Directly activated downstream targets of the WNT/beta-catenin pathway. Transcriptional target of beta-catenin/T-cell (Tcf) factor and may enhance cellular proliferation of tumor cells. Down-regulated by wild-type the adenomatosis polyposis coli (APC) protein. Elevated in many primary colon cancer tissues.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18p11.22
Cellular Component: integral to plasma membrane
Molecular Function: Wnt-protein binding
Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; hair follicle development; astrocyte cell migration
Disease: Hypotrichosis 1
Research Articles on APCDD1
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Product Notes
The APCDD1 apcdd1 (Catalog #AAA8115482) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 701aa. AAA Biotech's APCDD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the APCDD1 apcdd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly48 6. It is sometimes possible for the material contained within the vial of "APCDD1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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