Rabbit Bcl-rambo Polyclonal Antibody | anti-BCL2L13 antibody

Bcl-rambo Antibody

Cat. Num. AAA151034

• Gene Names: BCL2L13; MIL1; BCL-RAMBO; Bcl2-L-13
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Bcl-rambo Antibody is affinity chromatography purified via peptide column.
• Synonyms: Bcl-rambo; Polyclonal Antibody; Bcl-rambo Antibody; MIL1; BCL-RAMBO; Bcl2-L-13; CD003; Bcl-2-like protein 13; BCL2-like 13 (apoptosis facilitator); anti-BCL2L13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Bcl-rambo Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 485

• Applicable Applications for anti-BCL2L13 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo.
• Buffer: Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Bcl-rambo antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 μg/mL.)

Related Product Information for anti-BCL2L13 antibody

Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 14043326
• NCBI GeneID: 23786
• UniProt Accession #: Q9BXK5; Q96B37; Q96IB7; Q9BY01; Q9HC05; Q9UFE0; Q9UKN3; B3KPE7
• Molecular Weight: 34,601 Da
• NCBI Official Full Name: BCL2-like 13 (apoptosis facilitator)
• NCBI Official Synonym Full Names: BCL2-like 13 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L13
• NCBI Official Synonym Symbols: MIL1; BCL-RAMBO; Bcl2-L-13
• NCBI Protein Information: bcl-2-like protein 13
• UniProt Protein Name: Bcl-2-like protein 13
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L13
• UniProt Synonym Gene Names: MIL1; Bcl2-L-13
• UniProt Entry Name: B2L13_HUMAN

NCBI Description

This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Bcl-2L13: May promote the activation of caspase-3 and apoptosis. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrion; mitochondrial membrane; integral to membrane; nucleus

Molecular Function: caspase activator activity

Biological Process: caspase activation; apoptosis