SDS-Page
Complement C5 Active Protein | C3 active protein
Human CellExp Complement C5, Human Recombinant
Purity
>90% by SDS-PAGE
Synonyms
Complement C5; Human CellExp Complement C5; Human Recombinant; C5; CPAMD4; C3 active protein
Host
HEK293 cells
Purity/Purification
>90% by SDS-PAGE
Form/Format
Lyophilized powder. Lyophilized from 0.22um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Sequence
Gln19-Cys1676
Gene Source
Human
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Biological Activity
Immobilized Human Complement C5 at 2 ug/mL (100 uL/well) can bind Anti-C5a with a linear range of 0.2-3 ng/mL
Reconstitution Instruction
Reconstitute in sterile deionized water to the desired protein concentration.
Tag Note
This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain.
Preparation and Storage
Store at -20 degree C
Centrifuge the vial prior to opening.
Shelf life: ~12 months
Centrifuge the vial prior to opening.
Shelf life: ~12 months
SDS-Page
SDS-Page
Testing Data
Testing Data
Related Product Information for C3 active protein
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Product Categories/Family for C3 active protein
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated MW of 73.3kDa (beta chain) and 114.4kDa (alpha chain).
The protein migrates as 66kDa (beta chain) and 110kDa (alpha chain)under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein migrates as 66kDa (beta chain) and 110kDa (alpha chain)under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Family
Similar Products
Product Notes
The C3 (Catalog #AAA8400090) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Gln19-Cys1 676. It is sometimes possible for the material contained within the vial of "Complement C5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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