Rabbit WEE1 Polyclonal Antibody | anti-WEE1 antibody

WEE1 (Phospho-Ser53) Antibody

Cat. Num. AAA9503303

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: WEE1; Polyclonal Antibody; WEE1 (Phospho-Ser53) Antibody; anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: WEE1 (Phospho-Ser53) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser53.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-WEE1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:20000
• Target Modification: Phospho
• Modification Sites: H:S53; M:S52; R:S52
• Immunogen: The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53.
• Immunogen Range: 19-68
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 221307502
• NCBI GeneID: 7465
• NCBI Accession #: NP_001137448.1
• NCBI GenBank Nucleotide #: NM_001143976.1
• UniProt Accession #: P30291; B3KVE1; D3DQV0
• Molecular Weight: 71 kDa
• NCBI Official Full Name: wee1-like protein kinase isoform 2
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.