CD147 Active Protein | BSG active protein

CD147 Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122691

• Gene Names: Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD147; CD147 Protein; Mouse; Recombinant (His & hFc Tag); Mouse CD147/EMMPRIN/Basigin Protein (His & Fc Tag); AI115436 Protein; AI325119 Protein; EMMPRIN Protein; HT-7 Protein; basigin (Ok blood group); BSG active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg209

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 22
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse BSG isoform 2 (NP_001070652.1)(Met 1-Arg 209) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Using the Octet RED System. The affinity constant (Kd) of mouse CD147-Fc bound to Human PPIA-His was 0.4uM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BSG active protein

Background: CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

References

Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.

NCBI and Uniprot Product Information

• NCBI GI #: 116014342
• NCBI GeneID: 12215
• NCBI Accession #: NP_001070652.1
• NCBI GenBank Nucleotide #: NM_001077184.1
• UniProt Accession #: P18572; Q6LDB0; Q7TSC0
• Molecular Weight: 29,674 Da
• NCBI Official Full Name: basigin isoform 2
• NCBI Official Synonym Full Names: basigin
• NCBI Official Symbol: Bsg
• NCBI Official Synonym Symbols: HT-7; CD147; EMMPRIN; AI115436; AI325119
• NCBI Protein Information: basigin; 5A11/Basigin; HT7 antigen; basic immunoglobulin superfamily; gp 42; membrane glycoprotein gp42; neurothelin
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: Bsg
• UniProt Entry Name: BASI_MOUSE