IP (Immunoprecipitation)
(Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody AAA212649 (lot AAA212649-1) used for IP at 6 ug per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 antibody BL16383. For blotting immunoprecipitated B7-H3/CD276, A304-367A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human B7-H3/CD276 Polyclonal Antibody | anti-B7-H3/CD276 antibody
Rabbit anti-B7-H3/CD276 Antibody, Affinity Purified
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
B7-H3/CD276, Antibody; Rabbit anti-B7-H3/CD276 Antibody, Affinity Purified; B7RP-2; CD276 antigen; costimulatory molecule; CD antigen CD276; B7H3; 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276 molecule; anti-B7-H3/CD276 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-B7-H3/CD276 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 484 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody AAA212649 (lot AAA212649-1) used for IP at 6 ug per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 antibody BL16383. For blotting immunoprecipitated B7-H3/CD276, A304-367A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B7-H3/CD276 antibody AAA212649 (lot AAA212649-1) used for IP at 6 ug per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 antibody BL16383. For blotting immunoprecipitated B7-H3/CD276, A304-367A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human B7-H3/CD276 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. AAA212649 Lot 1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC hematoxylin)
IHC (Immunohiostchemistry)
(Detection of human B7-H3/CD276 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. AAA212649 Lot 1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC hematoxylin)
ICC (Immunocytochemistry)
(Detection of human B7-H3/CD276 by immunocytochemistry. Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. AAA212649 Lot 1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
ICC (Immunocytochemistry)
(Detection of human B7-H3/CD276 by immunocytochemistry. Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (Cat. No. AAA212649 Lot 1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-B7-H3/CD276 antibody
CD276 may participate in the regulation of T-cell-mediated immune response. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It may also be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces and could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5ZPR3].
Product Categories/Family for anti-B7-H3/CD276 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,235 Da
NCBI Official Full Name
CD276 antigen isoform a
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
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Product Notes
The B7-H3/CD276 cd276 (Catalog #AAA212649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-B7-H3/CD276 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H3/CD276 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the B7-H3/CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H3/CD276, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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