Rabbit anti-Human Oncostatin M/OSM Monoclonal Antibody | anti-OSM antibody

Recombinant Anti-Oncostatin M/OSM Antibody, Rabbit Monoclonal

Cat. Num. AAA8102858

• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: Oncostatin M/OSM; Monoclonal Antibody; Recombinant Anti-Oncostatin M/OSM Antibody; Rabbit Monoclonal; Oncostatin M/OSM Antibody; Rabbit MAb; oncostatin M; anti-OSM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 015
• Specificity: Human Oncostatin M/OSM.; No cross-reactivity in ELISA with Mouse OSM, Human CNTF, Human IL2, Human IL6, Human LIF, Human GCSF
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-OSM antibody: ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ELISA: 1:5000-1:10,000; ICC/IF: 1:20-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Oncostatin M/OSM protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Oncostatin M/OSM (rh Oncostatin M/OSM).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of OSM in jurkat cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OSM monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

Related Product Information for anti-OSM antibody

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeI and typeII both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produces the fully active 196 residue form.

References

Tanaka M, et al. (2003) Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. Reviews of Physiology, Biochemistry and Pharmacology. 149: 39-52.Auguste P, et al. (1997) Signaling of type II oncostatin M receptor. J Biol Chem. 272 (25): 15760-4.Zarling JM, et al. (1986). Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci. 83 (24): 9739-43.

NCBI and Uniprot Product Information

• NCBI GI #: 10092621
• NCBI GeneID: 5008
• NCBI Accession #: NP_065391.1
• NCBI GenBank Nucleotide #: NM_020530.4
• UniProt Accession #: P13725; Q6FHP8; Q9UCP6
• Molecular Weight: 28,484 Da
• NCBI Official Full Name: oncostatin-M preproprotein
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: OSM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• Protein Family: Oncostatin
• UniProt Gene Name: OSM
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_HUMAN

NCBI Description

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; oncostatin-M receptor binding; cytokine activity

Biological Process: multicellular organismal development; negative regulation of hormone secretion; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; cell proliferation; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; response to heat; positive regulation of acute inflammatory response; regulation of growth; positive regulation of cell division; negative regulation of meiosis; positive regulation of cell proliferation; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; immune response