Western Blot (WB)
(HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).)
Rabbit anti-Human, mouse HOMEZ Polyclonal Antibody | anti-HOMEZ antibody
HOMEZ Antibody (N-term)
Western Blot (WB)
(HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).)
Western Blot (WB)
(HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).)
Flow Cytometry (FC/FACS)
(HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.)
Bayarsaihan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 100(18):10358-10363(2003)
Ceulemans, H., et al. Bioessays 24(4):371-381(2002)
NCBI and Uniprot Product Information
Uniprot Description
HOMEZ: May function as a transcriptional regulator.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: DNA binding; transcription corepressor activity; transcription factor activity
Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Research Articles on HOMEZ
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Product Notes
The HOMEZ homez (Catalog #AAA9213521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 158-186. The HOMEZ Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HOMEZ can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the HOMEZ homez for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOMEZ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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