Lefty2 active protein

Lefty2 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120137

• Gene Names: LEFTY2; EBAF; LEFTA; TGFB4; LEFTYA
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Lefty2; Lefty2 Protein; Human; Recombinant (hFc Tag); Human Lefty2/Lefty A Protein (Fc Tag); EBAF Protein; LEFTA Protein; LEFTYA Protein; TGFB4 Protein; left-right determination factor 2; Lefty2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe78-Pro366

• Species: Human
• Activity: Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 4-16ug/mL in the presence of 50ng/mL of recombinant human BMP4.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mature form of human LeftyA (NP_003231.2)(Phe 78-Pro 366) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for Lefty2 active protein

Background: left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor-beta superfamily), and transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.

References

Feldman B, et al. (2002) Lefty antagonism of Squint is essential for normal gastrulation. Curr Biol. 12(24): 2129-35.Meno C, et al. (1999) Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. Mol Cell. 4(3): 287-98.Meno C, et al. (2001) Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. Dev Cell. 1(1): 127-38.

NCBI and Uniprot Product Information

• NCBI GI #: 27436881
• NCBI GeneID: 7044
• NCBI Accession #: NP_003231.2
• NCBI GenBank Nucleotide #: NM_003240.3
• UniProt Accession #: O00292; O75611; Q5TE89; Q8NBQ9; B3KNH4; B4E332; E9PDM4
• Molecular Weight: 37,093 Da
• NCBI Official Full Name: left-right determination factor 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: left-right determination factor 2
• NCBI Official Symbol: LEFTY2
• NCBI Official Synonym Symbols: EBAF; LEFTA; TGFB4; LEFTYA
• NCBI Protein Information: left-right determination factor 2; TGF-beta-4; protein lefty-2; protein lefty-A; left-right determination factor A; transforming growth factor beta-4; endometrial bleeding-associated factor
• UniProt Protein Name: Left-right determination factor 2
• Protein Family: Left-right determination factor
• UniProt Gene Name: LEFTY2
• UniProt Synonym Gene Names: EBAF; LEFTA; LEFTYA; TGFB4; TGF-beta-4
• UniProt Entry Name: LFTY2_HUMAN

NCBI Description

This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

LEFTY2: Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. Defects in LEFTY2 are the cause of left-right axis malformations (LRAM). The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava. Belongs to the TGF-beta family.

Protein type: Ligand, receptor tyrosine kinase; Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 1q42.1

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; platelet activation; platelet degranulation; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; multicellular organismal development; cell growth; blood coagulation; cell development