Typical Testing Data/Standard Curve (for reference only)
Human Involucrin ELISA Kit | INV elisa kit
Human Involucrin ELISA Kit
Reactivity
Human
Synonyms
Involucrin; Human Involucrin ELISA Kit; INV elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for INV elisa kit
Intended Uses: This IL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL. This ELISA kit for research use only!
Principle of the Assay: IL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL antibody and an IL-HRP conjugate. The assay sample and buffer are incubated together with IL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL concentration since IL from samples and IL-HRP conjugate compete for the anti-IL antibody binding site. Since the number of sites is limited, as more sites are occupied by IL from the sample, fewer sites are left to bind IL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL antibody and an IL-HRP conjugate. The assay sample and buffer are incubated together with IL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL concentration since IL from samples and IL-HRP conjugate compete for the anti-IL antibody binding site. Since the number of sites is limited, as more sites are occupied by IL from the sample, fewer sites are left to bind IL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL concentration in each sample is interpolated from this standard curve.
Product Categories/Family for INV elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,479 Da
NCBI Official Full Name
involucrin
NCBI Official Synonym Full Names
involucrin
NCBI Official Symbol
IVL
NCBI Protein Information
involucrin
UniProt Protein Name
Involucrin
Protein Family
UniProt Gene Name
IVL
UniProt Entry Name
INVO_HUMAN
NCBI Description
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
Uniprot Description
IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; cornified envelope
Molecular Function: protein binding, bridging; structural molecule activity
Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B
Research Articles on INV
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Product Notes
The Human INV ivl (Catalog #AAA723946) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723946 ELISA Kit recognizes Human INV. It is sometimes possible for the material contained within the vial of "Involucrin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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