Western Blot (WB)
(Western Blot: Sample: Recombinant a4GALT, Human.)
Rabbit anti-Human Alpha-1,4-Galactosyltransferase Polyclonal Antibody | anti-a4GALT antibody
Alpha-1,4-Galactosyltransferase (a4GALT) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant a4GALT, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant a4GALT, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. This protein, a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
Uniprot Description
A4GALT: Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins. Belongs to the glycosyltransferase 32 family.
Protein type: EC 2.4.1.228; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 22q13.2
Cellular Component: integral to Golgi membrane; membrane
Molecular Function: galactosyltransferase activity; lactosylceramide 4-alpha-galactosyltransferase activity; toxin binding
Biological Process: globoside biosynthetic process; glycosphingolipid biosynthetic process; plasma membrane organization; protein glycosylation
Disease: Blood Group, P1pk System
Research Articles on a4GALT
Similar Products
Product Notes
The a4GALT a4galt (Catalog #AAA2032238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alpha-1,4-Galactosyltransferase (a4GALT) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-1,4-Galactosyltransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the a4GALT a4galt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-1,4-Galactosyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
