EphB2 active protein

EphB2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123213

• Purity: >95% as determined by SDS-PAGE
• Synonyms: EphB2; EphB2 Protein; Mouse; Recombinant (His Tag); Mouse EphB2/Hek5 Protein (His Tag); Cek5 Protein; Drt Protein; Erk Protein; ETECK Protein; Hek5 Protein; Nuk Protein; Prkm5 Protein; Qek5 Protein; Sek3 Protein; Tyro5 Protein; EPH receptor B2; EphB2 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys540

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHB2 (NP_034272.1)(Met1-Lys540) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized mouse EPHB2-His at 10ug/mL (100uL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.01-0.03ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse EPHB2-His at 10 ug/mL (100 uL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.01-0.03 ug/mL.)

Related Product Information for EphB2 active protein

Background: Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Product Categories/Family for EphB2 active protein

Actin Dynamics Signaling Pathway

References

Zisch AH, et al. (1998) Complex formation between EphB2 and Src requires phosphorylation of tyrosine 611 in the EphB2 juxtamembrane region. Oncogene. 16 (20): 2657-70.Yu HH, et al. (2001) Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene. 20 (30): 3995-4006.Zisch AH, et al. (2000) Replacing two conserved tyrosines of the EphB2 receptor with glutamic acid prevents binding of SH2 domains without abrogating kinase activity and biological responses. Oncogene. 19 (2): 177-87.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant mouse EPHB2 comprises 533 amino acids and has a predicted molecular mass of 59.3kDa. The apparent molecular mass of the protein is approximately 65kDa in SDS-PAGE under reducing conditions.
• Protein Family: Ephrin type-B receptor