EphA4 active protein

EphA4 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122834

• Purity: >98% as determined by SDS-PAGE
• Synonyms: EphA4; EphA4 Protein; Mouse; Recombinant (His Tag); Mouse EphA4/HEK8 Protein (His Tag); 2900005C20Rik Protein; AI385584 Protein; Cek8 Protein; Hek8 Protein; rb Protein; Sek Protein; Sek1 Protein; Tyro1 Protein; EPH receptor A4; EphA4 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr547

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA4 at 2ug/ml (100uL/well) can bind mouse EFNA5 with a linear range of 1.28-32ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHA4 (NP_031962.2) extracellular domain (Met 1-Thr 547) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EphA4 active protein

Background: EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Product Categories/Family for EphA4 active protein

Actin Dynamics Signaling Pathway

References

Murai KK, et al. (2003) Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat Neurosci. 6(2): 153-60.Kullander K, et al. (2003) Role of EphA4 and EphrinB3 in local neuronal circuits that control walking. Science. 299(5614): 1889-92.Smith A, et al. (1997) The EphA4 and EphB1 receptor tyrosine kinases and ephrin-B2 ligand regulate targeted migration of branchial neural crest cells. Curr Biol. 7(8): 561-70.

NCBI and Uniprot Product Information

• Molecular Weight: The secreted recombinant mouse EPHA4 consists of 538 amino acids and has a predicted molecular mass of 60kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EPHA4 is approximately 65kDa.
• Protein Family: Ephrin type-A receptor