Rabbit anti-Human ILK Polyclonal Antibody | anti-ILK antibody

ILK (Phospho-Ser246) Antibody

Cat. Num. AAA9403494

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: ILK; Polyclonal Antibody; ILK (Phospho-Ser246) Antibody; ILK1; p59ILK; kinase ILK; anti-ILK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of ILK only when phosphorylated at serine 246.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 452

• Applicable Applications for anti-ILK antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK.
• Modification: Phospho-Ser246
• Target Name: ILK
• Preparation and Storage: Store at -20 degree C/1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-ILK antibody

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.Hannigan G.E., Nature 379:91-96(1996).Janji B., Oncogene 19:3069-3077(2000).Tadic B., Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.

Product Categories/Family for anti-ILK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 62420873
• NCBI GeneID: 3611
• NCBI Accession #: NP_001014794.1
• NCBI GenBank Nucleotide #: NM_001014794.2
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
• Molecular Weight: 36,467 Da
• NCBI Official Full Name: integrin-linked protein kinase isoform 1
• NCBI Official Synonym Full Names: integrin-linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: focal adhesion; costamere; protein complex; terminal button; intercellular junction; cytosol; nucleoplasm; sarcomere; membrane; cell soma; lamellipodium; cytoplasm; stress fiber; plasma membrane; dendritic shaft; cell junction

Molecular Function: integrin binding; protein serine/threonine kinase activity; signal transducer activity; protein binding; SH3 domain binding; protein kinase binding; ATP binding

Biological Process: protein heterooligomerization; negative regulation of smooth muscle cell proliferation; positive regulation of axon extension; positive regulation of transcription, DNA-dependent; cell-matrix adhesion; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of MAP kinase activity; myelin formation; positive regulation of cell proliferation; protein kinase B signaling cascade; fibril organization and biogenesis; negative regulation of neuron apoptosis; positive regulation of cell-matrix adhesion; cell cycle arrest; positive regulation of BMP signaling pathway; integrin-mediated signaling pathway; cell aging; positive regulation of dendrite morphogenesis; positive regulation of myoblast differentiation; myelination in the peripheral nervous system; establishment and/or maintenance of epithelial cell polarity; positive regulation of osteoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; ureteric bud branching; regulation of actin cytoskeleton organization and biogenesis; negative regulation of protein kinase activity; nerve development; neurite morphogenesis; positive regulation of phosphorylation; positive regulation of cell migration