Human cPLA2 ELISA Kit | cPLA2 elisa kit

Human cPLA2 (Cytosolic Phospholipase A2) ELISA Kit

Cat. Num. AAA2502149

• Gene Names: PLA2G4A; PLA2G4; cPLA2-alpha
• Reactivity: Human
• Synonyms: cPLA2; Human cPLA2 (Cytosolic Phospholipase A2) ELISA Kit; cPLA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human cPLA2. No significant cross-reactivity or interference between Human cPLA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human cPLA2 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 749

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for cPLA2 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of cPLA2 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human cPLA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human cPLA2. You can calculate the concentration of Human cPLA2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23943920
• NCBI GeneID: 5321
• NCBI Accession #: NP_077734.1
• NCBI GenBank Nucleotide #: NM_024420.2
• UniProt Accession #: P47712; Q29R80; B1AKG4
• Molecular Weight: 85,239 Da
• NCBI Official Full Name: cytosolic phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• NCBI Official Symbol: PLA2G4A
• NCBI Official Synonym Symbols: PLA2G4; cPLA2-alpha
• NCBI Protein Information: cytosolic phospholipase A2; cPLA2; calcium-dependent phospholipid-binding protein; lysophospholipase; phosphatidylcholine 2-acylhydrolase; phospholipase A2 group IVA
• UniProt Protein Name: Cytosolic phospholipase A2
• UniProt Gene Name: PLA2G4A
• UniProt Synonym Gene Names: CPLA2; PLA2G4; cPLA2
• UniProt Entry Name: PA24A_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

Uniprot Description

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - linoleic acid; Phospholipase; EC 3.1.1.5; EC 3.1.1.4; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; icosanoid metabolic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation