Fractalkine Recombinant Protein | CX3CL1 recombinant protein

Fractalkine Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123027

• Gene Names: Cx3cl1; CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Fractalkine; Fractalkine Protein; Mouse; Recombinant (His Tag); Mouse Fractalkine/CX3CL1 Protein (His Tag); AB030188 Protein; ABCD-3 Protein; AI848747 Protein; CX3C Protein; Cxc3 Protein; D8Bwg0439e Protein; Scyd1 Protein; chemokine (C-X3-C motif) ligand 1; CX3CL1 recombinant protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln25-Lys105

• Species: Mouse
• Predicted N Terminal: Gln 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Cx3cl1 (O35188)(Gln25-Lys105) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CX3CL1 recombinant protein

Background: Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine/CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine/CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.

Product Categories/Family for CX3CL1 recombinant protein

Neuroinflammation

References

Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.

NCBI and Uniprot Product Information

• NCBI GI #: 31982017
• NCBI GeneID: 20312
• NCBI Accession #: NP_033168.2
• NCBI GenBank Nucleotide #: NM_009142.3
• UniProt Accession #: O35188; O35933; Q91V44
• Molecular Weight: 42,099 Da
• NCBI Official Full Name: fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: Cx3cl1
• NCBI Official Synonym Symbols: CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• NCBI Protein Information: fractalkine; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; neurotactin; small inducible cytokine subfamily D, 1; small-inducible cytokine D1
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: Cx3cl1
• UniProt Synonym Gene Names: Cx3c; Fkn; Scyd1
• UniProt Entry Name: X3CL1_MOUSE

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: extracellular space; cell projection; cell surface; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: platelet activation; positive regulation of neuroblast proliferation; neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; positive regulation of cell adhesion; wound healing; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of cytokine secretion; microglial cell activation; chemotaxis; angiogenesis involved in wound healing; macrophage chemotaxis; leukocyte adhesive activation; negative regulation of vasoconstriction; regulation of inhibitory postsynaptic membrane potential; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of cell migration