c-MET Active Protein | MET active protein

c-MET Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122870

• Gene Names: Met; HGF; HGFR; Par4; c-Met; AI838057
• Purity: >92% as determined by SDS-PAGE
• Synonyms: c-MET; c-MET Protein; Mouse; Recombinant (hFc Tag); Mouse c-MET/HGFR Protein (Fc Tag); AI838057 Protein; c-Met Protein; HGF Protein; HGFR Protein; Par4 Protein; MET proto-oncogene; receptor tyrosine kinase; MET active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn929

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2ug/ml (100uL/well) can bind mouse HGFR with a linear range of 1.28-32ng/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 25 & Ser 307
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 ug/ml (100 ul/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml.)

Related Product Information for MET active protein

Background: Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Product Categories/Family for MET active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.

NCBI and Uniprot Product Information

• NCBI GI #: 146198696
• NCBI GeneID: 17295
• NCBI Accession #: NP_032617.2
• NCBI GenBank Nucleotide #: NM_008591.2
• UniProt Accession #: F8VQL0; P16056
• Molecular Weight: 153,546 Da
• NCBI Official Full Name: hepatocyte growth factor receptor
• NCBI Official Synonym Full Names: met proto-oncogene
• NCBI Official Symbol: Met
• NCBI Official Synonym Symbols: HGF; HGFR; Par4; c-Met; AI838057
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: C-methyltransferase
• UniProt Gene Name: Met
• UniProt Entry Name: F8VQL0_MOUSE

Uniprot Description

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SAAS SAAS020635

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. RuleBase RU000307Contains Sema domain. SAAS SAAS002909Contains protein kinase domain. SAAS SAAS008266

Caution: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Ensembl ENSMUSP00000079324 Ensembl ENSMUSP00000111102 Ensembl ENSMUSP00000111103