c-MET / HGFR recombinant protein

Recombinant Mouse c-MET / HGFR Protein (Fc tag) (Fc tag)

Cat. Num. AAA2546883

• Gene Names: Met; HGF; HGFR; Par4; c-Met; AI838057
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: c-MET / HGFR; Recombinant Mouse c-MET / HGFR Protein (Fc tag) (Fc tag); AI838057; HGF; HGFR; Par4; c-Met; c-MET / HGFR recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1379

• Application Notes: The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved alpha and beta subunits. Each alpha and beta together with the C-terminal Fc tag consists of 1146 amino acids and has a predicted molecular mass of 128 (alpha =32 + Fc tagged beta=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Glu 25 & Ser 307
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for c-MET / HGFR recombinant protein

Background: Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotype s. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Description: A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 146198696
• NCBI GeneID: 17295
• NCBI Accession #: NP_032617.2
• NCBI GenBank Nucleotide #: NM_008591.2
• UniProt Accession #: P16056; Q62125
• Molecular Weight: 153,549 Da
• NCBI Official Full Name: hepatocyte growth factor receptor
• NCBI Official Synonym Full Names: met proto-oncogene
• NCBI Official Symbol: Met
• NCBI Official Synonym Symbols: HGF; HGFR; Par4; c-Met; AI838057
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• UniProt Gene Name: Met
• UniProt Synonym Gene Names: HGF receptor; SF receptor

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Met family; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 6 A2|6 7.83 cM

Cellular Component: basal plasma membrane; cell soma; cell surface; cytoplasm; dendrite; excitatory synapse; extracellular space; membrane; neuron projection; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: beta-catenin binding; hepatocyte growth factor receptor activity; phosphoinositide 3-kinase binding; phospholipase binding; protein complex binding; protein heterodimerization activity; protein kinase activity; protein phosphatase binding; protein-tyrosine kinase activity

Biological Process: activation of MAPK activity; adult behavior; brain development; branching morphogenesis of a tube; cardiac muscle cell development; cardiac muscle contraction; endothelial cell morphogenesis; glucose homeostasis; hepatocyte growth factor receptor signaling pathway; liver development; muscle cell migration; muscle development; myoblast proliferation; myotube differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron migration; placenta development; positive chemotaxis; positive regulation of dendrite morphogenesis; positive regulation of DNA replication; positive regulation of mitosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; regulation of excitatory postsynaptic membrane potential; regulation of interleukin-6 production; regulation of synaptic transmission; skeletal muscle development; sperm motility; synaptic transmission