Testing Data
Rabbit GSPT1 Polyclonal Antibody | anti-GSPT1 antibody
Anti-GSPT1 Antibody
Gene Names
GSPT1; GST1; ETF3A; eRF3a; 551G9.2
Reactivity
Mouse, Rat, Human. Not yet tested in other species.
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
GSPT1; Polyclonal Antibody; Anti-GSPT1 Antibody; anti-GSPT1 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human. Not yet tested in other species.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
633
Applicable Applications for anti-GSPT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA titer: 1:5,000-1:20,000
Western blot analysis: 1:500-1,000
Immunohistochemistry: 1:100-200
Optimal dilutions/concentrations should be determined by the end user.
Western blot analysis: 1:500-1,000
Immunohistochemistry: 1:100-200
Optimal dilutions/concentrations should be determined by the end user.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal eRF3/GSPT1 antibody was raised against a 16 amino acid peptide from N terminal residues of human eRF3/GSPT1 protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
References
(1) Malta-Vacas,J., Chauvin,C., Goncalves,L., et al.eRF3a/GSPT1 12-GGC allele increases the susceptibility for breast cancer development.Oncol. Rep. 21 (6), 1551-1558 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,700 Da
NCBI Official Full Name
GSPT1 protein
NCBI Official Synonym Full Names
G1 to S phase transition 1
NCBI Official Symbol
GSPT1
NCBI Official Synonym Symbols
GST1; ETF3A; eRF3a; 551G9.2
NCBI Protein Information
eukaryotic peptide chain release factor GTP-binding subunit ERF3A; G1 to S phase transition protein 1 homolog; eukaryotic peptide chain release factor subunit 3a
UniProt Protein Name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Protein Family
UniProt Gene Name
GSPT1
UniProt Entry Name
ERF3A_HUMAN
Uniprot Description
GSPT1 iso3: Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Belongs to the GTP-binding elongation factor family. ERF3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Translation; RNA-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 16p13.1
Cellular Component: intracellular
Molecular Function: GTPase activity; protein binding; GTP binding; translation release factor activity
Biological Process: protein amino acid methylation; mRNA catabolic process, nonsense-mediated decay; translational termination; G1/S transition of mitotic cell cycle
Research Articles on GSPT1
Similar Products
Product Notes
The GSPT1 gspt1 (Catalog #AAA416267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GSPT1 Antibody reacts with Mouse, Rat, Human. Not yet tested in other species. and may cross-react with other species as described in the data sheet. AAA Biotech's GSPT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA titer: 1:5,000-1:20,000 Western blot analysis: 1:500-1,000 Immunohistochemistry: 1:100-200 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the GSPT1 gspt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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