Prostatic Acid Phosphatase Cell Lysate | ACPP cell lysate
Mouse Prostatic Acid Phosphatase HEK293 Overexpression Lysate
Gene Names
Acpp; Lap; PAP; FRAP; Ppal; 5'-NT; A030005E02Rik
Applications
Western Blot
Synonyms
Prostatic Acid Phosphatase; Mouse Prostatic Acid Phosphatase HEK293 Overexpression Lysate; Mouse Prostatic Acid Phosphatase/ACPP HEK293 Cell Lysate (WB positive control); acid phosphatase; prostate; Mouse 5'-NT Overexpression Lysate; Mouse 9.104100899-104272570.1 Overexpression Lysate; Mouse A030005E02Rik Overexpression Lysate; Mouse FRAP Overexpression Lysate; Mouse Lap Overexpression Lysate; Mouse PAP Overexpression Lysate; Mouse Ppal Overexpression Lysate; ACPP cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
361aa
Sequence
Met1-Arg381
Applicable Applications for ACPP cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ACPP cell lysate
Prostatic acid phosphatase (PAP, or ACPP), also known as prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic acid phosphatase is synthesized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic acid phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His(12) and Asp(258)) that are located in the cleft between two domains. Cellular prostatic acid phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5'-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.
This Mouse Prostatic Acid Phosphatase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Prostatic Acid Phosphatase protein from the overexpression lysate was verified.
This Mouse Prostatic Acid Phosphatase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Prostatic Acid Phosphatase protein from the overexpression lysate was verified.
Product Categories/Family for ACPP cell lysate
References
Hassan MI, et al. (2010) Structural and functional analysis of human prostatic acid phosphatase. Expert Rev Anticancer Ther. 10(7): 1055-68.Chuang TD, et al. (2010) Human prostatic acid phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth. J Biol Chem. 285(31): 23598-606.Larsen RS, et al. (2009) A high throughput assay to identify small molecule modulators of prostatic acid phosphatase. Curr Chem Genomics. 3: 42-9.Zimmermann H. (2009) Prostatic acid phosphatase, a neglected ectonucleotidase. Purinergic Signal. 5(3): 273-5.Wang Y, et al. (2005) Prostatic acid phosphatase as a target molecule in specific immunotherapy for patients with nonprostate adenocarcinoma. J Immunother. 28(6): 535-41.Veeramani S, et al. (2005) Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer. 12(4): 805-22.Ostrowski WS, et al. (1994) Human prostatic acid phosphatase: selected properties and practical applications. Clin Chim Acta. 226(2): 121-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,863 Da
NCBI Official Full Name
prostatic acid phosphatase isoform 2
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
Acpp
NCBI Official Synonym Symbols
Lap; PAP; FRAP; Ppal; 5'-NT; A030005E02Rik
NCBI Protein Information
prostatic acid phosphatase
UniProt Protein Name
Prostatic acid phosphatase
Protein Family
UniProt Gene Name
Acpp
UniProt Synonym Gene Names
5'-NT; FRAP; TMPase
UniProt Entry Name
PPAP_MOUSE
Uniprot Description
ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.2; EC 3.1.3.5
Cellular Component: apical part of cell; extracellular region; extracellular space; filopodium; Golgi cisterna; integral to membrane; intracellular; lysosomal membrane; lysosome; membrane; multivesicular body; nucleus; plasma membrane; secretory granule; vesicle membrane
Molecular Function: 5'-nucleotidase activity; acid phosphatase activity; choline binding; hydrolase activity; identical protein binding; phosphoric monoester hydrolase activity; protein homodimerization activity; thiamin phosphate phosphatase activity
Biological Process: adenosine metabolic process; dephosphorylation; nucleotide metabolic process; positive regulation of adenosine receptor signaling pathway; purine base metabolic process; regulation of sensory perception of pain; thiamin metabolic process
Research Articles on ACPP
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Product Notes
The ACPP acpp (Catalog #AAA8116995) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 361aa. AAA Biotech's Prostatic Acid Phosphatase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ACPP acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg38 1. It is sometimes possible for the material contained within the vial of "Prostatic Acid Phosphatase, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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