Leucine Rich Repeat Kinase 2 (LRRK2) Recombinant Protein | LRRK2 recombinant protein

Recombinant Leucine Rich Repeat Kinase 2 (LRRK2)

Cat. Num. AAA2018399

• Gene Names: Lrrk2; Gm927; cI-46; AW561911; 4921513O20Rik; 9330188B09Rik; D630001M17Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Leucine Rich Repeat Kinase 2 (LRRK2); Recombinant Leucine Rich Repeat Kinase 2 (LRRK2); LRRK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- LLNGGCREQD VRKALTVSIQ KGDSQVISLL LRKLALDLAN NSICLGGFGI GKIDPSWLGP LFPDKSSNLR KQTNTGSVLA RKVLRYQMRN TLQEGVASGS DGKFSEDALA KFGEWTFIPD SSMDSVFGQS DDLDSEGSES SFLVKRKSNS ISVGEVYRDL ALQRCSPNAQ RHSNSLGPVF DHEDLLRRKR KILSSDESLR SSRLPSHMRQ SDSSSSLASE REHITSLDLS ANEL
• Sequence Length: 2527

• Applicable Applications for LRRK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu761~Leu994 (Accession # Q5S006) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 146231954
• NCBI GeneID: 66725
• NCBI Accession #: NP_080006.3
• NCBI GenBank Nucleotide #: NM_025730.3
• UniProt Accession #: Q5S006; Q8BWG7; Q8BZJ6; Q8CI84; Q8K062; E9QNJ2
• Molecular Weight: 57.7kDa
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 2
• NCBI Official Symbol: Lrrk2
• NCBI Official Synonym Symbols: Gm927; cI-46; AW561911; 4921513O20Rik; 9330188B09Rik; D630001M17Rik
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: Lrrk2
• UniProt Entry Name: LRRK2_MOUSE

Uniprot Description

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; LRRK family

Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; mitochondrion; lysosome; dendrite; terminal button; inclusion body; trans-Golgi network; ribonucleoprotein complex; cytosol; lipid raft; mitochondrial outer membrane; synaptic vesicle; cell soma; membrane; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; synapse; intracellular; cytoplasmic vesicle; endosome; neuron projection; intracellular membrane-bound organelle; endoplasmic reticulum; perikaryon; cell projection; axon; mitochondrial membrane; plasma membrane; cell junction

Molecular Function: tubulin binding; GTPase activity; identical protein binding; clathrin binding; GTP-dependent protein kinase activity; protein homodimerization activity; Rho GTPase binding; GTP binding; nucleotide binding; actin binding; GTPase activator activity; protein kinase activity; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; SNARE binding; protein kinase A binding; transferase activity, transferring phosphorus-containing groups; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding

Biological Process: regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; positive regulation of protein binding; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; small GTPase mediated signal transduction; regulation of dopamine receptor signaling pathway; cell differentiation; negative regulation of protein binding; regulation of mitochondrial depolarization; Golgi organization and biogenesis; positive regulation of autophagy; neuromuscular junction development; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; MAPKKK cascade; peptidyl-threonine phosphorylation; endocytosis; regulation of locomotion; positive regulation of programmed cell death; peptidyl-serine phosphorylation; regulation of membrane potential; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; positive regulation of protein amino acid phosphorylation; response to oxidative stress; regulation of excitatory postsynaptic membrane potential; phosphorylation; neurite morphogenesis; positive regulation of GTPase activity