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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP94 antibody.)

anti-Human GRP94 Antibody | anti-GRP94 antibody

GRP94 Antibody

Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
GRP94; GRP94 Antibody; TRA1; GLUCOSE-REGULATED PROTEIN 94-KD; STRESS-INDUCIBLE TUMOR REJECTION ANTIGEN GP96; TUMOR REJECTION ANTIGEN 1; tumor rejection antigen (gp96) 1; anti-GRP94 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
GRP94 antibody detects endogenous levels of total GRP94 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
803
Applicable Applications for anti-GRP94 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from human GRP94.
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP94 antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP94 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from SKOV3 cells, using GRP94 antibody.)

Western Blot (WB) (Western blot analysis of extracts from SKOV3 cells, using GRP94 antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HepG2 cells, using GRP94 antibody.)

Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HepG2 cells, using GRP94 antibody.)
References
Robyn C. Reed, J. Biol. Chem., Jul 2002; 277: 25082-25089. Julie C. Baker-LePain, J. Exp. Med., Dec 2002; 196: 1447. Péter Csermely, J. Biol. Chem., Mar 1995; 270: 6381. Robyn C. Reed, J. Biol. Chem., Aug 2003; 278: 31853-31860.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
92,469 Da
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90 beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94

NCBI Description

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Uniprot Description

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

Research Articles on GRP94

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Product Notes

The GRP94 hsp90b1 (Catalog #AAA854669) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The GRP94 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRP94 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the GRP94 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP94, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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