DLL-4, soluble Active Protein | DLL-4 active protein

Human DLL-4, soluble

Cat. Num. AAA692106

• Gene Names: DLL4; hdelta2
• Purity: > 95%, by SDS-PAGE and HPLC analysis
• Synonyms: DLL-4; soluble; Human DLL-4; Recombinant Human sDLL-4; hdelta2; DLL-4 active protein

• Host: HEK 293 Cells
• Purity/Purification: > 95%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized form 1X PBS, pH 7.5
• Sequence: SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPG PCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS L IIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTST LTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SC LPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECI PH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GAT CSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDG Y HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACEC PPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSI DACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP
• Sequence Length: 685

• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug).
• Reconstitution: Centrifuge vial prior to reopening.; Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex.
• Biological Activity: The sDLL-4, when immobilized at concentrations >1.5 ug/mL will inhibit myogenesis in C2C12 cells.
• Length (aa): 498
• Preparation and Storage: Reconstituted working aliquots are stable for 1 week at 2°C to 8°C. For 3 months at -20°C to -80°C.

Related Product Information for DLL-4 active protein

Human sDLL4 comprises the extracellular signaling domain of DLL, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. DLL4 functions to specifically activate the Notch-1 and Notch-4 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. Targeted deletion of the DLL4 gene in mice resulted in severe vascular defects and death before birth. Up-regulation of DLL4 expression has been implicated in the vascular development of certain tumors. Recombinant human sDLL4 is a 54.0 kDa glycoprotein containing 498 amino-acid residues.

Product Categories/Family for DLL-4 active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 9506545
• NCBI GeneID: 54567
• NCBI Accession #: NP_061947.1
• NCBI GenBank Nucleotide #: NM_019074.3
• UniProt Accession #: Q9NR61; Q3KP23; Q9NQT9
• Molecular Weight: 54 kDa
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta-like 4 (Drosophila)
• NCBI Official Symbol: DLL4
• NCBI Official Synonym Symbols: hdelta2
• NCBI Protein Information: delta-like protein 4; delta4; delta 4; delta ligand 4; notch ligand DLL4; delta-like 4 homolog; delta-like 4 protein; notch ligand delta-2; drosophila Delta homolog 4
• UniProt Protein Name: Delta-like protein 4
• UniProt Gene Name: DLL4
• UniProt Synonym Gene Names: Delta4
• UniProt Entry Name: DLL4_HUMAN

NCBI Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4

By similarity. Ref.8

Subunit structure: Binds to Notch-1 and Notch-4

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

Probable.

Tissue specificity: Expressed in vascular endothelium.

Domain: The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.

Post-translational modification: Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation

By similarity.

Sequence similarities: Contains 1 DSL domain.Contains 8 EGF-like domains.