DLL4 / Delta4 recombinant protein

Recombinant Human DLL4 / Delta4 Protein (His tag)

Cat. Num. AAA2546457

• Gene Names: DLL4; AOS6; hdelta2
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: DLL4 / Delta4; Recombinant Human DLL4 / Delta4 Protein (His tag); DLL4; MGC126344; hdelta2; DLL4 / Delta4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 685

• Application Notes: The recombinant human DLL4 consists of 509 amino acids and predicts a molecular mass of 55.7 kDa. As a result of glycosylation, the apparent molecular mass of rhDLL4 is approximately 55-70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DLL4 / Delta4 recombinant protein

Background: Delta-like protein 4 (DLL4, Delta4), a type I membrane-bound Notch ligand, is one of five known Notch ligands in mammals and interacts predominantly with Notch 1, which has a key role in vascular development. Recent studies yield substantial insights into the role of DLL4 in angiogenesis. DLL4 is induced by vascular endothelial growth factor (VEGF) and acts downstream of VEGF as a 'brake' on VEGF-induced vessel growth, forming an autoregulatory negative feedback loop inactivating VEGF. DLL4 is downstream of VEGF signaling and its activation triggers a negative feedback that restrains the effects of VEGF. Attenuation of DLL4/Notch signaling results in chaotic vascular network with excessive branching and sprouting. DLL4 is widely distributed in tissues other than vessels including many malignancies. Furthermore, the molecule is internalized on binding its receptor and often transported to the nucleus. In pathological conditions, such as cancer, DLL4 is up-regulated strongly in the tumour vasculature. Blockade of DLL4-mediated Notch signaling strikingly increases nonproductive angiogenesis, but significantly inhibits tumor growth in preclinical mouse models. In preclinical studies, blocking of DLL4/Notch signaling is associated with a paradoxical increase in tumor vessel density, yet causes marked growth inhibition due to functionally defective vasculature. Thus, DLL4 blockade holds promise as an additional strategy for angiogenesis-based cancer therapy.

Description: A DNA sequence encoding the extracellular domain (Met 1-Pro 524) of human DLL4 (NP_061947.1) pro-protein was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 9506545
• NCBI GeneID: 54567
• NCBI Accession #: NP_061947.1
• NCBI GenBank Nucleotide #: NM_019074.3
• UniProt Accession #: Q9NR61; Q3KP23; Q9NQT9
• Molecular Weight: 74,605 Da
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 4
• NCBI Official Symbol: DLL4
• NCBI Official Synonym Symbols: AOS6; hdelta2
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• UniProt Gene Name: DLL4
• UniProt Synonym Gene Names: Delta4

NCBI Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: plasma membrane

Molecular Function: protein binding

Biological Process: angiogenesis; blood circulation; blood vessel remodeling; negative regulation of cell proliferation; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; Notch receptor processing; Notch signaling pathway; patterning of blood vessels; positive regulation of Notch signaling pathway; signal transduction; T cell differentiation

Disease: Adams-oliver Syndrome 6