Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit | PTGS2 elisa kit

Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit

Cat. Num. AAA2702306

• Gene Names: PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Reactivity: Rat
• Synonyms: Prostaglandin Endoperoxide Synthase 2 (PTGS2); Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit; PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2).; No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.
• Sequence Length: 604

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1020089
• NCBI GeneID: 5743
• UniProt Accession #: P35354; Q16876; A8K802
• Molecular Weight: 68,996 Da
• NCBI Official Full Name: prostaglandin endoperoxide synthase-2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2
• NCBI Official Symbol: PTGS2
• NCBI Official Synonym Symbols: COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• NCBI Protein Information: prostaglandin G/H synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: PTGS2
• UniProt Synonym Gene Names: COX2; COX-2; PGH synthase 2; PGHS-2

NCBI Description

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.