Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 ELISA Kit | HCN4 elisa kit

Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 ELISA Kit

Cat. Num. AAA761257

• Gene Names: HCN4; SSS2
• Reactivity: Human
• Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 ELISA Kit; HCN4 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4)/hyperpolarization activated cyclic nucleotide-gated cation channel 4/hyperpolarization activated cyclic nucleotide-gated potassium channel 4; HCN4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection ofHCN4. No significant cross-reactivity or interference betweenHCN4and analogues was observed.
• Sequence Length: 1203

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelHCN4were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelHCN4were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HCN4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HCN4antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HCN4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HCN4amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHCN4can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38605641
• NCBI GeneID: 10021
• Molecular Weight: 129,042 Da
• NCBI Official Full Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• NCBI Official Synonym Full Names: hyperpolarization activated cyclic nucleotide gated potassium channel 4
• NCBI Official Symbol: HCN4
• NCBI Official Synonym Symbols: SSS2
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• Protein Family: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel
• UniProt Gene Name: HCN4
• UniProt Entry Name: HCN4_HUMAN

NCBI Description

This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15. [provided by RefSeq, Oct 2008]

Uniprot Description

HCN4: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2); also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder. Defects in HCN4 are the cause of Brugada syndrome type 8 (BRGDA8). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the potassium channel HCN family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, cation

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: integral to membrane; intrinsic to plasma membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: cAMP binding; identical protein binding; intracellular cAMP activated cation channel activity; voltage-gated potassium channel activity; voltage-gated sodium channel activity

Biological Process: blood circulation; cation transport; muscle contraction; regulation of heart rate; regulation of membrane potential; synaptic transmission

Disease: Brugada Syndrome 8; Sick Sinus Syndrome 2, Autosomal Dominant