Human Hyaluronidase-4 ELISA Kit | HYAL4 elisa kit

Human Hyaluronidase-4 ELISA Kit

Cat. Num. AAA764377

• Gene Names: HYAL4; CSHY
• Reactivity: Human
• Synonyms: Hyaluronidase-4; Human Hyaluronidase-4 ELISA Kit; HYAL4/Hyaluronidase 4/CSHY/chondroitin sulfate hydrolase/CSHY/Hyal-4/hyaluronidase-4/Hyaluronoglucosaminidase-4; HYAL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HYAL4 . No significant cross-reactivity or interference between HYAL4 and analogues was observed.
• Sequence Length: 481

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HYAL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HYAL4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HYAL4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HYAL4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HYAL4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HYAL4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HYAL4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 166235888
• NCBI GeneID: 23553
• NCBI Accession #: NP_036401.2
• NCBI GenBank Nucleotide #: NM_012269.2
• Molecular Weight: 54,249 Da
• NCBI Official Full Name: hyaluronidase-4
• NCBI Official Synonym Full Names: hyaluronoglucosaminidase 4
• NCBI Official Symbol: HYAL4
• NCBI Official Synonym Symbols: CSHY
• NCBI Protein Information: hyaluronidase-4
• UniProt Protein Name: Hyaluronidase-4
• Protein Family: Hyaluronidase
• UniProt Gene Name: HYAL4
• UniProt Synonym Gene Names: Hyal-4; CSHY
• UniProt Entry Name: HYAL4_HUMAN

NCBI Description

This gene encodes a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

HYAL4: Endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. Has also chondroitin sulfate hydrolase activity, The best substrate being the galactosaminidic linkage in the sequence of a trisulfated tetrasaccharide. Belongs to the glycosyl hydrolase 56 family.

Protein type: EC 3.2.1.35; Membrane protein, integral; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: cell surface; integral to membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: carbohydrate metabolic process; chondroitin sulfate catabolic process; glycosaminoglycan catabolic process